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Preliminary molecular detection of the somatic embryogenesis receptor-like kinase (VpSERK) and knotted-like homeobox (VpKNOX1) genes during in vitro morphogenesis of Vanilla planifolia Jacks

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Abstract

This work aimed to evaluate the embryogenic competence of different tissues from different stages (friable callus, bud-regenerating callus, and whole buds) of Vanilla planifolia, through the molecular detection of the somatic embryogenesis receptor-like kinase (VpSERK) and knotted-like homeobox (VpKNOX1) genes. RNA was extracted with Trizol®, cDNA was obtained, and the studied transcripts were amplified. Using non-specific primers, VpSERK and VpSTM gene expression was detected in the three stages evaluated. This study might contribute to providing an explanation for the recalcitrance of this Vanilla species to somatic embryogenesis.

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Acknowledgements

The authors thank the “Programa para el Desarrollo Profesional Docente (PRODEP)” for financial support provided for the project “Biotechnological Basis for the Genetic Improvement of Vanilla planifolia” within the “Conservation, Management and Plant Breeding network”. MARM thanks the Consejo Nacional de Ciencia y Tecnología (CONACyT) for a grant scholarship No. 275736 which allowed the realization of this work. We thank Jaime A. Teixeira da Silva for revising the manuscript, improving the English and offering critical analysis of the scientific content.

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Authors and Affiliations

  1. Instituto de Biotecnología y Ecología Aplicada (INBIOTECA), Universidad Veracruzana, Av. de las Culturas Veracruzanas No. 101, Campus para la Cultura, las Artes y el Deporte, Col. Emiliano Zapata, 91090, Xalapa, Veracruz, Mexico

    Marco A. Ramírez-Mosqueda & Lourdes G. Iglesias-Andreu

  2. Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Colonia Chuburná de Hidalgo, Mérida, Yucatán, Mexico

    Luis Sáenz & Iván Córdova

Authors
  1. Marco A. Ramírez-Mosqueda
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  2. Lourdes G. Iglesias-Andreu
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  3. Luis Sáenz
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  4. Iván Córdova
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Corresponding authors

Correspondence to Marco A. Ramírez-Mosqueda or Lourdes G. Iglesias-Andreu.

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Ramírez-Mosqueda, M.A., Iglesias-Andreu, L.G., Sáenz, L. et al. Preliminary molecular detection of the somatic embryogenesis receptor-like kinase (VpSERK) and knotted-like homeobox (VpKNOX1) genes during in vitro morphogenesis of Vanilla planifolia Jacks. 3 Biotech 8, 94 (2018). https://doi.org/10.1007/s13205-018-1112-7

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  • DOI: https://doi.org/10.1007/s13205-018-1112-7

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