Abstract
The present study optimized the extraction protocol conditions to obtain the antioxidant-rich bioactive extracts from Carica papaya L. (Papaya) leaves. A Box and Behnken Design (BBD) consisting of three different extracting variables viz. extracting temperature (60–70 °C), time (10–20 min) and solvent concentration (55–65%) was used. Antioxidant efficacy was recorded by evaluating four responses viz. ABTS, DPPH, SASA and total phenolic contents. The optimized model predicted, solvent concentration of 60% with extraction time of 15 min and extracting temperature of 65 °C with bioactive-rich antioxidants having highest total phenolic activity. The efficacy of obtained bioactive-rich papaya leaves extracts (PLE) were subjected for in-vivo evaluation in chevon emulsion added with the level of T-1 (0.10%); T-2 (0.25%); T-3 (0.50%) and control (without extract) stored under refrigeration (4 ± 1 °C) for 9 days by evaluating various physicochemical, microbiological, sensory quality characteristics. The pH was significantly higher for control than all treatments and water activity (aw) showed decreasing trend throughout storage period. Oxidation efficiency values showed an increasing drift during storage period, irrespective of added level of PLE, showing lowest oxidation in samples treated with 0.5% PLE. Sensory panellists awarded comparatively higher scores to all PLE treatments than control. Microbiological quality of emulsion incorporated with different levels of papaya leaves extract successfully improved and was lower in 0.5% PLE treated samples. It was concluded that extraction of bioactive antioxidants from Carica papaya L. leaves improved by optimising extraction parameters using RSM. Carica papaya L. leaves extracts have proven prospects as natural anti-oxidants in chevon emulsion as a meat emulsion system.
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References
APHA (2001) Compendium of methods for the microbiological examination of food, 4th edn. In: Speck ML (ed) American Public Health Association. Washington
Asase A, Akwetey GA, Achel DG (2010) Ethnopharmacological use of herbal remedies for the treatment of malaria in the Dangme West District of Ghana. J Ethnopharmacol 129:367–376. https://doi.org/10.1016/j.jep.2010.04.001
Beriain MJ, Gomez I, Ibanez FC, Sarries MV, Ordonez AI (2018) Improvement of the functional and healthy properties of meat products. Food Qual Balanc Health Dis. https://doi.org/10.1016/B978-0-12-811442-1.00001-8
Biswas AK, Chatli MK, Sahoo J (2012) Antioxidant potential of curry (Murraya koenigii L.) and mint (Mentha spicata) leaf extracts and their effect on colour and oxidative stability of raw ground pork meat during refrigeration storage. Food Chem 133(2):467–472. https://doi.org/10.1016/j.foodchem.2012.01.073
Botsoglou E, Govaris A, Ambrosiadis I, Fletouris D, Botsoglou N (2014) Effect of olive leaf (Olea europea L.) extracts on protein and lipid oxidation of long-term frozen n-3 fatty acids-enriched pork patties. Meat Sci 98:150–157. https://doi.org/10.1016/j.meatsci.2014.05.015
Demeyer A, Verplaetse M, Gistelinck K (1986) Fermentation of meat: an integrated process. In: 32nd European meat research work, Ghent, Belgium, pp 241–246. http://agris.fao.org/agris-search/search.do?recordID=BE19870068741. Accessed 7 June 2018
Devatkal SK, Kumboj R, Paul D (2014) Comparative antioxidant effect of BHT and water extracts of banana and sapodilla peels in raw poultry meat. J Food Sci Technol 51(2):387–391. https://doi.org/10.1007/s13197-011-0508-8
FAO (2016) FAO statistical yearbook, Rome, Italy. http://www.fao.org/faostat/en/#data/QA. Accessed 7 June 2018
GOI (2012) Government of India: 19th livestock census-2012, Department of Animal Husbandry, Dairying and Fisheries, Ministry of Agriculture, Krishi Bhawan, New Delhi, India
Greene BE, Cumuze TH (1982) Relationship between TBA numbers and inexperienced panelists assessments of oxidized flavor in cooked beef. J Food Sci 47(1):52–54. https://doi.org/10.1111/j.1365-2621.1982.tb11025.x
Haenlein GFW (2004) Goat milk in human nutrition. Small Rumin Res 51(2):155–163. https://doi.org/10.1016/j.smallrumres.2003.08.010
Jimenez-Colmenero F (2007) Healthier lipid formulation approaches in meat-based functional foods. Technological options for replacement of meat fats by non-meat fats. Trends Food Sci Technol 18(11):567–578. https://doi.org/10.1016/j.tifs.2007.05.006
Joseph S, Chatli MK, Biswas AK, Sahoo J (2014) Efficacy of pink guava pulp as an antioxidant in raw pork emulsion. J Food Sci Technol 51(8):1492–1500. https://doi.org/10.1007/s13197-012-0668-1
Kagan VE (1988) Lipid peroxidation in biomembranes. CRC Press, Boca Raton. https://www.taylorfrancis.com/books/9781351082532. Accessed 7 June 2018
Kato K, Terao S, Shimamoto N, Hirata M (1988) Studies on scavengers of active oxygen species: synthesis and biological activity of 2-o-alkylascorbic acids. J Med Chem 31(4):793–798. https://doi.org/10.1021/jm00399a019
Keeton JT (1983) Effect of fat and sodium chloride phosphate levels on the chemical and sensory properties of pork patties. J Food Sci 36:261–276. https://doi.org/10.1111/j.1365-2621.1983.tb14921.x
Koniecko R (1979) Handbook for meat chemists. Avery Publishing Group, Inc. Wayne, pp 53–55. http://agris.fao.org/agris-search/search.do?recordID=US8818945. Accessed 7 June 2018
Kumar V, Chatli MK, Wagh RV, Mehta N, Kumar P (2015) Effect of the combination of natural antioxidants and packaging methods on quality of pork patties during storage. J Food Sci Technol 52(10):6230–6241. https://doi.org/10.1007/s13197-015-1734-2
Mancini RA, Hunt MC (2005) Current research in meat color. Meat Sci 71:100–121. https://doi.org/10.1016/j.meatsci.2005.03.003
Miean KH, Mohamed S (2001) Flavonoid (myricetin, quercetin, kaempferol, luteolin, and apigenin) content of edible tropical plants. J Agric Food Chem 49:3106–3112. https://doi.org/10.1021/jf000892m
Noor S, Bhat ZF, Kumar S, Mudiyanselage RJ (2018) Preservative effect of Asparagus racemosus: a novel additive for bioactive edible films for improved lipid oxidative stability and storage quality of meat products. Meat Sci 139:207–212. https://doi.org/10.1016/j.meatsci.2018.02.001
Pietrasik Z, Dhanda JS, Shand PJ, Pegg RB (2006) Influence of injection, packaging, and storage conditions on the quality of beef and bison steaks. J Food Sci 71(2):110–118. https://doi.org/10.1111/j.1365-2621.2006.tb08913.x
Realini CE, Guadia MD, Diaz I, Garcia-Regueiro JA, Arnau J (2015) Effects of Acerola fruit extract on sensory and shelf-life of salted beef patties from grinds differing in fatty acid composition. Meat Sci 99:18–24. https://doi.org/10.1016/j.meatsci.2014.08.008
Shirwaikar A, Prabhu K, Punitha ISR (2006) In-vitro antioxidant studies of Sphaeranthus indicus. Indian J Exp Biol 44:993–998. http://nopr.niscair.res.in/handle/123456789/6666. Accessed 7 June 2018
Silva EM, Rogez H, Larondelle Y (2007) Optimization of extraction of phenolics from Inga edulis leaves using response surface methodology. Sep Purif Technol 55:381–387. https://doi.org/10.1016/j.seppur.2007.01.008
Sorwar MG, Mostofa M, Hasan MN, Billah M, Rahman MT (2016) Effect of kalo jeera seeds and papaya leaf supplementation on the performance of broiler. Bangladesh J Vet Med 14(1):37–42. https://doi.org/10.3329/bjvm.v14i1.28820
Wagh RV, Chatli MK (2017) Response surface optimization of extraction protocols to obtain phenolic rich antioxidant from sea buckthorn and their potential application into model meat system. J Food Sci Technol 54(6):1565–1576. https://doi.org/10.1007/s13197-017-2588-6
Wagh RV, Chatli MK, Kumar V (2014) Comparative Efficacy of Different Matteuccia Struthiopteris extracts as an Antioxidant in Raw Ground Pork During Refrigerated Storage. Haryana Veterinarian. 53(2):102–105
Wagh RV, Chatli MK, Ruusunen R, Puolanne E, Ertbjerg P (2015) Effect of various phyto-extracts on physico-chemical, colour, and oxidative stability of pork frankfurters. Asian Australas J Anim Sci 28(8):1178–1186. https://doi.org/10.5713/ajas.14.0752
Witte VC, Krause GF, Bailey ME (1970) A new extraction method for determining 2-thiobarbituric acid values of pork beef during storage. J Food Sci 35:582–585. https://doi.org/10.1111/j.1365-2621.1970.tb04815.x
Yogiraj V, Goyal PK, Chauhan CS, Goyal A, Vyas B (2014) Carica papaya Linn: an overview. Int J Herb Med 2(5):1–8. http://www.florajournal.com/archives/2014/vol2issue5/PartA/2-4-12.1.pdf. Accessed 7 June 2018
Yuan YV, Bone DE, Carrington MF (2005) Antioxidant activity of dulse (Palmaria palmata) extract evaluated in-vitro. Food Chem 91:485–494. https://doi.org/10.1016/j.foodchem.2004.04.039
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Authors highly acknowledge the support and laboratory facilities provided by the Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India. This research did not receive any specific funding.
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Jagtap, N.S., Wagh, R.V., Chatli, M.K. et al. Optimisation of extraction protocol for Carica papaya L. to obtain phenolic rich phyto-extract with prospective application in chevon emulsion system. J Food Sci Technol 56, 71–82 (2019). https://doi.org/10.1007/s13197-018-3456-8
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DOI: https://doi.org/10.1007/s13197-018-3456-8