Skip to main content
Log in

Physicochemical characterization, antioxidant and anticancer activities of proteins from four legume species

  • Original Article
  • Published:
Journal of Food Science and Technology Aims and scope Submit manuscript

Abstract

The physicochemical, functional, antioxidant and anticancer properties of protein isolates from the seeds of Soybean (SP), Black soybean (BSP), Adzuki bean (ABP), and Mung bean (MBP) were comparatively characterized. The difference was found in the protein composition and physicochemical properties of the four types of legume proteins, which affected the functional properties and bioactivities. BSP and SP had six predominant proteins with a molecular weight (MW) range of 20–95 kDa, whereas ABP and MBP showed the most intense bands of 48 kDa. ABP with higher essential amino acids content and the highest solubility exhibited the highest antioxidant activities among the four types of proteins. While BSP with higher content of acidic amino acids, low content of the hydrophobic amino acids and higher WHC, may have potential nutraceutical uses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • AOAC Intl (1995) Official methods of analysis of AOAC international. AOAC Intl (p. v. (loose-leaf)), Arlington, Va

    Google Scholar 

  • Barac MB, Pesic MB, Stanojevic SP, Kostic AZ, Bivolarevic V (2015) Comparative study of the functional properties of three legume seed isolates: adzuki, pea and soy bean. J Food Sci Technol 52:2779–2787

    Article  CAS  Google Scholar 

  • Barbana C, Boye JI (2013) In vitro protein digestibility and physico-chemical properties of flours and protein concentrates from two varieties of lentil (Lens culinaris). Food Funct 4:310–321

    Article  CAS  Google Scholar 

  • Chan YS, Xia L, Ng TB (2016) White kidney bean lectin exerts anti-proliferative and apoptotic effects on cancer cells. Int J Biol Macromol 85:335–345

    Article  CAS  Google Scholar 

  • Garcia-Mora P, Frias J, Peñas E, Zieliński H, Giménez-Bastida JA, Wiczkowski W, Zielińskac D, Martínez-Villaluenga C (2015) Simultaneous release of peptides and phenolics with antioxidant, ACE-inhibitory and anti-inflammatory activities from pinto bean (Phaseolus vulgaris L. var. pinto) proteins by subtilisins. J Funct Foods 18:319–332

    Article  CAS  Google Scholar 

  • Ghiselli A, Nardini M, Baldi A, Scaccini C (1998) Antioxidant activity of different phenolic fractions separated from an Italian red wine. J Agric Food Chem 46:361–367

    Article  CAS  Google Scholar 

  • Ghribi AM, Gafsi IM, Sila A, Blecker C, Danthine S, Attia H, Bougatef A, Besbes S (2015) Effects of enzymatic hydrolysis on conformational and functional properties of chickpea protein isolate. Food Chem 187:322–330

    Article  Google Scholar 

  • Guan X, Yao H, Chen Z, Shan L, Zhang M (2007) Some functional properties of oat bran protein concentrate modified by trypsin. Food Chem 101:163–170

    Article  CAS  Google Scholar 

  • Kudre TG, Benjakul S, Kishimura H (2013) Comparative study on chemical compositions and properties of protein isolates from mung bean, black bean and bambara groundnut. J Sci Food Agric 93:2429–2436

    Article  CAS  Google Scholar 

  • Li W, Shu C, Yan S, Shen Q (2010) Characteristics of sixteen mung bean cultivars and their protein isolates. Int J Food Sci Technol 45:1205–1211

    Article  CAS  Google Scholar 

  • Liu H, Liu H, YanL ChengX, Kang Y (2015) Functional properties of 8S globulin fractions from 15 mung bean (Vigna radiata(L.) Wilczek) cultivars. Int J Food Sci Technol 50:1206–1214

    Article  CAS  Google Scholar 

  • Misurcova L, Bunka F, Ambrozova JV, Machu L, Samek D, Kracmar S (2014) Amino acid composition of algal products and its contribution to RDI. Food Chem 151:120–125

    Article  CAS  Google Scholar 

  • Pan WL, Ng TB (2015) A dimeric Phaseolus coccineus lectin with anti-oxidative, anti-proliferative and cytokine-inducing activities. Int J Biol Macromol 81:960–966

    Article  CAS  Google Scholar 

  • Rayaprolu SJ, Hettiarachchy NS, Chen P, Kannan A, Mauromostakos A (2013) Peptides derived from high oleic acid soybean meals inhibit colon, liver and lung cancer cell growth. Food Res Int 50:282–288

    Article  CAS  Google Scholar 

  • Shevkani K, Kaur A, Kumar S, Singh N (2015) Cowpea protein isolates: functional properties and application in gluten-free rice muffins. LWT Food Sci Technol 63:927–933

    Article  CAS  Google Scholar 

  • Stone AK, Karalash A, Tyler RT, Warkentin TD, Nickerson MT (2015) Functional attributes of pea protein isolates prepared using different extraction methods and cultivars. Food Res Int 76:31–38

    Article  CAS  Google Scholar 

  • Tan ES, Ngoh YY, Gan CY (2014) A comparative study of physicochemical characteristics and functionalities of pinto bean protein isolate (PBPI) against the soybean protein isolate (SP) after the extraction optimisation. Food Chem 152:447–455

    Article  CAS  Google Scholar 

  • Tang CH, Sun X (2011) A comparative study of physicochemical and conformational properties in three vicilins from Phaseolus legumes: implications for the structure–function relationship. Food Hydrocolloids 25:315–324

    Article  CAS  Google Scholar 

  • Tjahjadi C, Lin S, Breene WM (1988) Isolation and characterization of Adzuki bean (Vigna angularis cv Takara) proteins. J Food Sci Technol 53:1438–1443

    CAS  Google Scholar 

  • Wang J, Zhang Q, Zhang Z, Li Z (2008) Antioxidant activity of sulfated polysaccharide fractions extracted from Laminaria japonica. Int J Biol Macromol 42:127–132

    Article  Google Scholar 

  • Wani IA, Sogi DS, Shivhare US, Gill BS (2015) Physico-chemical and functional properties of native and hydrolyzed kidney bean (Phaseolus vulgaris L.) protein isolates. Food Res Int 76:11–18

    Article  CAS  Google Scholar 

  • WHO (1985) Energy and protein requirements. Report of a joint FAO/WHO/UNU expert consultation. Geneva, World Health Organization, WHO Technical Report Series, No. 724

  • Yu X, Yuan F, Fu X, Zhu D (2016) Profiling and relationship of water-soluble sugar and protein compositions in soybean seeds. Food Chem 196:776–782

    Article  CAS  Google Scholar 

  • Zhang M, Tang X, Wang F, Zhang Q, Zhang Z (2013) Characterization of Lycium barbarum polysaccharide and its effect on human hepatoma cells. Int J Biol Macromol 61:270–275

    Article  CAS  Google Scholar 

  • Zhang Y, Chen HX, Zhang N, Ma LS (2015) Antioxidant and functional properties of tea protein as affected by the different tea processing methods. J FoodSci Technol 52:742–752

    Article  CAS  Google Scholar 

  • Zhou DY, Zhu BW, Qiao L, Wu HT, Li DM, Yang JF, Murata Y (2012) In vitro antioxidant activity of enzymatic hydrolysates prepared from abalone (Haliotis discus hannai Ino) viscera. Food Bioprod Process 90:148–154

    Article  CAS  Google Scholar 

  • Zhu LJ, Chen J, Tang XY, Xiong YL (2008) Reducing, radical scavenging, and chelation properties of in vitro digests of alcalase-treated zein hydrolysate. J Agric Food Chem 56:2714–2721

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the grant from National High Technology Research and Development Program (“863” Program) of China (Grant No. SS2013AA100207) and the National Natural Science Foundation of China (NSFC 31371879).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Haixia Chen.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chen, Z., Wang, J., Liu, W. et al. Physicochemical characterization, antioxidant and anticancer activities of proteins from four legume species. J Food Sci Technol 54, 964–972 (2017). https://doi.org/10.1007/s13197-016-2390-x

Download citation

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13197-016-2390-x

Keywords

Navigation