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Effect of removal of phenolic compounds on structural and thermal properties of sunflower protein isolate

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Abstract

The present study evaluated the effect of removal of polyphenols on the structural properties of protein isolates extracted from sunflower seed and kernel. The structural and thermal changes in protein upon phenolic interaction were studied using circular dichroism, differential scanning calorimetry, thermal gravimetric analysis, X-ray diffraction, sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE), and Fourier Transform Infrared (FT-IR) spectroscopy. Presence of phenolic compounds in proteins decreased the ordered structure content with parallel increase in unordered structure content. Denaturation temperature was higher for protein isolates with phenolic compounds while, enthalpy decreased upon phenolic interaction. In the presence of phenolic compounds, higher mass loss was observed upon heating. Crystalinity and crystal size got increased after removal of phenolic compounds. Protein isolates from kernels had higher percentage of crystalinity and crystal size as compared to seed protein isolates. Higher molecular weights were observed for protein isolates with phenolic compounds. Presence of polyphenols reduced the hydrophobicity as well the sulfhydryl content and increased the particle size of proteins.

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References

  • Adochitei A, Drochioiu G (2011) Rapid characterization of peptide secondary structure by FTIR spectroscopy. Rev Roum Chim 56:783–791

    CAS  Google Scholar 

  • Aewsiri T, Benjakul S, Visessanguan W, Eun JB, Wierenga PA, Gruppen H (2009) Antioxidative activity and emulsifying properties of cuttlefish skin gelatin modified by oxidised phenolic compounds. Food Chem 117:160–168

    Article  CAS  Google Scholar 

  • Ali M, Homann T, Khalil M, Kruse HP, Rawel H (2013) Milk whey protein modification by coffee-specific phenolics: effect on structural and functional properties. J Agric Food Chem 61:6911–6920

    Article  CAS  Google Scholar 

  • Alvarez LM (2014) Honey proteins and their interaction with polyphenols. Brock University. http://dr.library.brocku.ca/handle/10464/3415. Accessed 26 Jan 2016

  • Benjakul S, Seymour TS, Morrissey MT, An H (1997) Physico-chemical changes in Pacific whiting muscle proteins during iced storage. J Food Sci 62:729–733

    Article  CAS  Google Scholar 

  • Cao Y, Tan HM (2005) Study on crystal structures of enzyme-hydrolyzed cellulosic materials by X-ray diffraction. Enzym Microb Technol 36:314–317

    Article  CAS  Google Scholar 

  • Chandrapala J, Zisu B, Palmer M, Kentish S, Ashokkumar M (2011) Effects of ultrasound on the thermal and structural characteristics of proteins in reconstituted whey protein concentrate. Ultrason Sonochem 18:951–957

    Article  CAS  Google Scholar 

  • Dorrell DG, Vick BA (1997) Properties and processing of oilseed sunflower. In: Schneiter AA (ed) Sunflower technology and production. American Society of Agronomy, Madison, pp 709–744

    Google Scholar 

  • Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70–77

    Article  CAS  Google Scholar 

  • Gassmann B (1983) Preparation and application of vegetable proteins, especially proteins from sunflower seed for human consumption: an approach. Food/Nahrung 27:351–369

    Article  CAS  Google Scholar 

  • González-Pérez S, Vereijken JM (2007) Sunflower proteins: overview of their physicochemical, structural and functional properties. J Sci Food Agric 87:2173–2191

    Article  Google Scholar 

  • González-Pérez S, Merck KB, Vereijken JM, Van Koningsveld GA, Gruppen H, Voragen AGJ (2002) Isolation and characterization of undenatured chlorogenic acid-free sunflower (Helianthus annuus) proteins. J Agric Food Chem 50:1713–1719

    Article  Google Scholar 

  • Hagenmaier FD (1974) Aqueous processing of full-fat sunflower seeds: yields of oil and protein. J Am Oil Chem Soc 51:470–471

    Article  CAS  Google Scholar 

  • Hasni I, Bourassa P, Hamdani S, Samson G, Carpentier R, Tajmir-Riahi HA (2011) Interaction of milk α- and β-caseins with tea polyphenols. Food Chem 126:630–639

    Article  CAS  Google Scholar 

  • Hayakawa S, Nakai S (1985) Relationships of hydrophobicity and net charge to the solubility of milk and soy proteins. J Food Sci 50:486–491

    Article  CAS  Google Scholar 

  • Jia Z, Zheng M, Tao F, Chen W, Huang G, Jiang J (2016) Effect of covalent modification by (-)-epigallocatechin-3-gallate on physicochemical and functional properties of whey protein isolate. LWT Food Sci Technol 66:305–310

    Article  CAS  Google Scholar 

  • Joshi M, Adhikari B, Aldred P, Panozzo JF, Kasapis S (2011) Physicochemical and functional properties of lentil protein isolates prepared by different drying methods. Food Chem 129:1513–1522

    Article  CAS  Google Scholar 

  • Kanakis CD, Hasni I, Bourassa P, Hamdani S, Tarantilis PA, Tajmir-Riahi HA (2011) Milk β-lactoglobulin complexes with tea polyphenols. Food Chem 127:1046–1055

    Article  CAS  Google Scholar 

  • Kroll J, Rawel HM, Rohn S (2003) Reactions of plant phenolics with food proteins and enzymes under special consideration of covalent bonds. Food Sci Technol Res 9:205–218

    Article  CAS  Google Scholar 

  • Kumar KS, Ganesan K, Selvaraj K, Rao PVS (2014) Studies on the functional properties of protein concentrate of Kappaphycus alvarezii (Doty) Doty—An edible seaweed. Food Chem 153:353–360

    Article  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  CAS  Google Scholar 

  • Murray ED, Arntfield SD, Ismond MAH (1985) The influence of processing parameters on food protein functionality. II. Factor affecting thermal properties as analyzed by differential scanning calorimetry. Can Inst Food Sci Technol J 18:158–162

    Article  CAS  Google Scholar 

  • Pawar VD, Patil JN, Sakhale BK, Agarkar BS (2001) Studies on selected functional properties of defatted sunflower meal and its high protein products. J Food Sci Technol 38:47–51

    CAS  Google Scholar 

  • Prigent SVE, Gruppen H, Visser AJWG, Van-Koningsveld GAHD, Alfons GJV (2003) Effects of non-covalent interactions with 5-o-caffeoylquinic acid (CGA) on the heat denaturation and solubility of globular proteins. J Agric Food Chem 51:5088–5095

    Article  CAS  Google Scholar 

  • Rawel HM, Czajka D, Rohn S, Kroll J (2002a) Interactions of different phenolic acids and flavonoids with soy proteins. Int J Biol Macromol 30:137–150

    Article  CAS  Google Scholar 

  • Rawel HM, Rohn S, Kruse HP, Kroll J (2002b) Structural changes induced in bovine serum albumin by covalent attachment of chlorogenic acid. Food Chem 78:443–455

    Article  CAS  Google Scholar 

  • Rohn S, Rawel HM, Rober M, Kroll J (2005) Reactions with phenolic substances can induce changes in some physico-chemical properties and activities of bromelain—The consequences for supplementary food products. Int J Food Sci Technol 40:771–782

    Article  CAS  Google Scholar 

  • Salgado PR, Ortiz SEM, Petruccelli S, Mauri AN (2011) Sunflower protein concentrates and isolates prepared from oil cakes have high water solubility and antioxidant capacity. J Am Oil Chem Soc 88:351–360

    Article  CAS  Google Scholar 

  • Shevkani K, Singh N (2015) Relationship between protein characteristics and film-forming properties of kidney bean, field pea and amaranth protein isolates. Int J Food Sci Technol 50:1033–1043

    Article  CAS  Google Scholar 

  • Shevkani K, Singh N, Rana JC, Kaur A (2014) Relationship between physicochemical and functional properties of amaranth (Amaranthus hypochondriacus) protein isolates. Int J Food Sci Technol 49:541–550

    Article  CAS  Google Scholar 

  • Shevkani K, Singh N, Kaur A, Rana JC (2015) Structural and functional characterization of kidney bean and field pea protein isolates: a comparative study. Food Hydrocoll 43:679–689

    Article  CAS  Google Scholar 

  • Shimada K, Cheftel JC (1988) Determination of sulfhydryl groups and disulphide bonds in heat-induced gels of soy protein isolate. J Agric Food Chem 36:147–153

    Article  CAS  Google Scholar 

  • Song NB, Song HY, Jo WS, Song KB (2013) Physical properties of a composite film containing sunflower seed meal protein and its application in packaging smoked duck meat. J Food Eng 116:789–795

    Article  CAS  Google Scholar 

  • Wang Q, Du Y, Hu X, Yang J, Fan L, Feng T (2006) Preparation of alginate/soy protein isolate blend fibers through a novel coagulating bath. J Appl Polym Sci 101:425–431

    Article  CAS  Google Scholar 

  • Yao F, Wu Q, Lei Y, Guo W, Xu Y (2008) Thermal decomposition kinetics of natural fibers: activation energy with dynamic thermogravimetric analysis. Polym Degrad Stab 93:90–98

    Article  CAS  Google Scholar 

  • Yu L, Yang W, Sun J, Zhang C, Bi J, Yang Q (2015) Preparation, characterisation and physicochemical properties of the phosphate modified peanut protein obtained from Arachin Conarachin L. Food Chem 170:169–179

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Food Engineering and Technology, SLIET, Longowal, Sangrur, Punjab, 148106, India

    M. A. Malik, H. K. Sharma & C. S. Saini

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  1. M. A. Malik
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  2. H. K. Sharma
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  3. C. S. Saini
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Correspondence to C. S. Saini.

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Malik, M.A., Sharma, H.K. & Saini, C.S. Effect of removal of phenolic compounds on structural and thermal properties of sunflower protein isolate. J Food Sci Technol 53, 3455–3464 (2016). https://doi.org/10.1007/s13197-016-2320-y

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  • DOI: https://doi.org/10.1007/s13197-016-2320-y

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