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Cloning and characterization of mersacidin like bacteriocin from Bacillus licheniformis MKU3 in Escherichia coli

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Abstract

A putative gene encoding mersacidin like lantibiotic bacteriocin (lanA) was identified in Bacillus licheniformis genome. The lanA open reading frame codes for 74 amino acids with calculated isoelectric point of 6.7 and molecular mass of 8.2 kDa. The lanA gene was amplified from B. licheniformis MKU3, cloned in pQE30 vector and overexpressed in Escherichia coli M15. The recombinant peptide was purified to homogeneity using Ni–NTA chromatography and the SDS-PAGE analysis of the purified peptide revealed it to be a monomer with molecular mass of ~8.5 kDa. The purified bacteriocin showed wide spectrum activity against gram-positive pathogens. The peptide was found to be stable under in wide range of pH, temperature tolerant and resistant to the proteolytic enzymes. The stable nature of the bacteriocin to high temperature and resistant to various chemicals it also exhibited antimicrobial activity against food-borne pathogens make this bacteriocin as potent attractive antimicrobial agent in food products.

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Acknowledgments

Authors gratefully acknowledge the Council of Scientific Industrial Research (CSIR) New Delhi, India for the financial assistance through New Millennium Indian Technology Leadership Initiative (5/258/13/2002-NMITLI, 5/258/13A/2005-TNBD) and the Award of DST WOS-A, New Delhi, India for the financial assistance through Women Scientist Scheme (SR/WOS-A/LS-629/2012).

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Authors and Affiliations

  1. Department of Zoology, Periyar University, Salem, 636 011, Tamil Nadu, India

    Nagarajan Kayalvizhi

  2. Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India

    Neelamegam Rameshkumar

  3. Department of Genetics, School of Biological Sciences, Madurai Kamaraj University, Madurai, 625 021, Tamil Nadu, India

    Paramasamy Gunasekaran

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  1. Nagarajan Kayalvizhi
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  2. Neelamegam Rameshkumar
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  3. Paramasamy Gunasekaran
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Correspondence to Paramasamy Gunasekaran.

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Kayalvizhi, N., Rameshkumar, N. & Gunasekaran, P. Cloning and characterization of mersacidin like bacteriocin from Bacillus licheniformis MKU3 in Escherichia coli . J Food Sci Technol 53, 2298–2306 (2016). https://doi.org/10.1007/s13197-016-2195-y

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  • DOI: https://doi.org/10.1007/s13197-016-2195-y

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