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Properties and characteristics of nanocomposite films from tilapia skin gelatin incorporated with ethanolic extract from coconut husk

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Abstract

Impacts of ethanolic extract from coconut husk (EECH) at 0–0.4 % (w/w, on protein basis) on properties of films from tilapia skin gelatin and gelatin/Cloisite Na+ nanocomposite films were investigated. Young’s Modulus, tensile strength and elongation at break of both films decreased with addition of EECH (P < 0.05). The lowest water vapour permeability (WVP) was obtained for gelatin film containing 0.05 % EECH (w/w) (P < 0.05). Nevertheless, the nanocomposite film showed the lowest WVP when incorporated with 0.4 % EECH (w/w) (P < 0.05). Generally, L*- value (lightness) decreased and a*- value (redness) of films increased (P < 0.05) with increasing levels of EECH, regardless of nanoclay incorporation. Transparency of both films generally decreased as the level of EECH increased (P < 0.05). Intercalated or exfoliated structure of nanocomposite films was revealed by wide angle X-ray diffraction (WAXD) analysis. Based on scanning electron microscopic (SEM) analysis, the rougher surface was found when EECH was added. EECH had varying impact on thermal stability of films as revealed by thermogravimetric (TGA) and differential scanning calorimetric (DSC) analyses. Thus, the incorporation of EECH determined the properties of both gelatin film and nanocomposite film in which the improved water vapour barrier property could be obtained.

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References

  • Abdollahi M, Rezaei M, Farzi G (2012) A novel active bionanocomposite film incorporating rosemary essential oil and nanoclay into chitosan. J Food Eng 111:343–350

    Article  CAS  Google Scholar 

  • AOAC (2000) Official methods of analysis, 17th edn. Association of Official Analytical Chemists, Gaithersberg

    Google Scholar 

  • ASTM (1989) Standard test methods for water vapor transmission of materials (E96-E80). In: Annual book of ASTM standards. ASTM, Philadelphia. 730–739

  • Avena-Bustillos RJ, Krochta JM (1993) Water vapor permeability of caseinate-based edible films as affected by pH, calcium crosslinking and lipid content. J Food Sci 58:904–907

    Article  CAS  Google Scholar 

  • Bae HJ, Park HJ, Hong SI, Byun YJ, Darby DO, Kimmel RM, Whiteside WS (2009) Effect of clay content, homogenisation RPM, pH, and ultrasonification on mechanical and barrier properties of fish gelatin/montmorillonite nanocomposite films. LWT Food Sci Technol 42:1179–1186

    Article  CAS  Google Scholar 

  • Bao S, Xu S, Wang Z (2009) Antioxidant activity and properties of gelatin films incorporated with tea polyphenol-loaded chitosan nanoparticles. J Sci Food Agric 89:2692–2700

    Article  CAS  Google Scholar 

  • Bitencourt CM, Favaro-Trindade CS, Sobral PJA, Carvalho RA (2014) Gelatin-based films additivated with curcuma ethanol extract: antioxidant activity and physical properties of films. Food Hydrocoll 40:145–152

    Article  CAS  Google Scholar 

  • Chinabhark K, Benjakul S, Prodpran T (2007) Effect of pH on the properties of protein-based film from bigeye snapper (Priacanthus tayenus) surimi. Bioresour Technol 98:221–225

    Article  CAS  Google Scholar 

  • Farahnaky A, Mohammad S, Dadfar M, Shahbazi M (2014) Physical and mechanical properties of gelatin–clay nanocomposite. J Food Eng 122:78–83

    Article  CAS  Google Scholar 

  • Gennadios A, Brandenburg AH, Weller CL, Testin RF (1993) Effect of pH on properties of wheat gluten and soy protein isolate films. J Agric Food Chem 41:1835–1839

    Article  CAS  Google Scholar 

  • Gennadios A, Weller CL, Hanna MA, Froning GW (1996) Mechanical and barrier properties of egg albumen films. J Food Sci 61:585–589

    Article  CAS  Google Scholar 

  • Gomez-Estaca J, Lopez-de-Dicastillo C, Hernandez-Munoz P, Catala R, Gavara R (2014) Advances in antioxidant active food packaging. Trends Food Sci Technol 35:42–51

    Article  CAS  Google Scholar 

  • Gomez-Guillen MC, Porez-Mateos M, Gomez-Estaca J, Lopez-Caballero E, Gimenez B, Montero P (2009) Fish gelatin: a renewable material for developing active biodegradable films. Trends Food Sci Technol 20:3–16

    Article  CAS  Google Scholar 

  • Grevellec JI, Marquie C, Ferry L, Crespy A, Vialettes V (2001) Processability of cottonseed proteins into biodegradable materials. Biomacromolecules 2:1104–1109

    Article  CAS  Google Scholar 

  • Gutierrez MQ, Echeverria I, Ihl M, Bifani V, Mauri AN (2012) Carboxymethylcellulose–montmorillonite nanocomposite films activated with murta (Ugni molinae Turcz) leaves extract. Carbohydr Polym 87:1495–1502

    Article  Google Scholar 

  • Hamaguchi PY, WuYin W, Tanaka M (2007) Effect of pH on the formation of edible films made from the muscle proteins of blue marlin (Makaira mazara). Food Chem 100:914–920

    Article  CAS  Google Scholar 

  • Han JH, Krochta JM (1999) Water vapor permeability and wetting properties of whey protein coating on paper. AM Soc Agric Eng 42:1375–1382

    Article  CAS  Google Scholar 

  • Haslam E (1998) Practical polyphenolics. From structure to molecular recognition and physiological action. Cambridge University Press, Cambridge

    Google Scholar 

  • Hoque MS, Benjakul S, Prodpran T (2011) Properties of film from cuttlefish (Sepia pharaonis) skin gelatin incorporated with cinnamon, clove and star anise extracts. Food Hydrocoll 25:1085–1097

    Article  CAS  Google Scholar 

  • Iwata KI, Ishizaki SH, Handa AK, Tanaka MU (2000) Preparation and characterisation of edible films from fish water-soluble proteins. Fish Sci 66:372–378

    Article  CAS  Google Scholar 

  • Jongjareonrak A, Benjakul S, Visessanguan W, Tanaka M (2008) Antioxidant activity and properties of skin gelatin films incorporated with BHT and α-tocopherol. Food Hydrocoll 22:449–458

    Article  CAS  Google Scholar 

  • Karim AA, Bhat R (2009) Fish gelatin: properties, challenges, and prospects as an alternative to mammalian gelatin. Food Hydrocoll 23:563–576

    Article  CAS  Google Scholar 

  • Kavoosi G, Dadfar SMM, Purfard AM, Mehrabi R (2013) Antioxidant and antibacterial properties of gelatin films incorporated with carvacrol. J Food Saf 33:423–432

    Article  Google Scholar 

  • Koh MJ, Hwang HY, Kim DJ, Kim HJ, Hong YT, Nam SY (2010) Preparation and characterization of porous PVdF-HFP/clay nanocomposite membranes. J Mater Sci Technol 26:633–638

    Article  CAS  Google Scholar 

  • Li P, Zheng JP, Ma YL, Yao KD (2003) Gelatin/Montmorillonite hybrid nanocomposite. II Swelling behavior. J Applied Polym Sci 88:322–326

    Article  CAS  Google Scholar 

  • Li J, Miao J, Wu J, Chen S, Zhang Q (2014) Preparation and characterization of active gelatin-based films incorporated with natural antioxidants. Food Hydrocoll 37:166–173

    Article  CAS  Google Scholar 

  • Martucci JF, Ruseckaite RA (2008) Structure and properties of gelatin/montomorillonite nanocomposite films. In: Jimenez A, Zaikov GE (eds) Recent advances in research on biodegradable polymers and sustainable polymers. Nova Publishers, Huntington, pp 27–36

    Google Scholar 

  • Martucci JF, Ruseckaite RA (2010a) Biodegradable bovine gelatin/Na+-montmorillonite nanocomposite films. Structure, barrier and dynamic mechanical properties. Polym -Plast Technol Eng 49:581–588

    Article  CAS  Google Scholar 

  • Martucci JF, Ruseckaite RA (2010b) Biodegradable three-layer film derived from bovine gelatin. J Food Eng 99:377–383

    Article  CAS  Google Scholar 

  • Martucci JF, Vazquez A, Ruseckaite RA (2007) Nanocomposites based on gelatin and montmorillonite. Morphological and thermal studies. J Therm Anal Calorim 89:117–122

    Article  CAS  Google Scholar 

  • Nagarajan M, Benjakul S, Prodpran T, Songtipya P (2014a) Characteristics of bio-nanocomposite films from tilapia skin gelatin incorporated with hydrophilic and hydrophobic nanoclays. J Food Eng 143:195–204

    Article  CAS  Google Scholar 

  • Nagarajan M, Benjakul S, Prodpran T, Songtipya P (2014b) Properties of bio-nanocomposite films from tilapia skin gelatin as affected by different nanoclays and homogenising conditions. Food Bioprocess Technol 7:3269–3281

    Article  CAS  Google Scholar 

  • Nunez-Flores R, Gimenez B, Fernandez-Martin F, Lopez-Caballero ME, Montero MP, Gomez-Guillen MC (2012) Role of lignosulphonate in properties of fish gelatin films. Food Hydrocoll 27:60–71

    Article  CAS  Google Scholar 

  • Nuthong P, Benjakul S, Prodpran T (2009) Characterization of porcine plasma protein-based films as affected by pretreatment and cross-linking agents. Int J Biol Macromol 44:143–148

    Article  CAS  Google Scholar 

  • Oxford P, Parker R, Ring S (1989) Effect of water as a diluent on the glass transition behaviour of malto-oligosaccharides, amylase and amylopectin. Int J Biol Macromol 11:91–96

    Article  Google Scholar 

  • Papadopoulou A, Frazier RA (2004) Characterisation of protein–polyphenol interactions. Trends Food Sci Technol 15:186–190

    Article  CAS  Google Scholar 

  • Pradhan NK, Das M, Palve YP, Nayak PL (2012) Synthesis and characterization of soya protein isolate/cloisite 30B (MMT) nanocomposite for controlled release of anticancer drug curcumin. Int J Res Pharm Biomed Sci 3:1513–1522

    CAS  Google Scholar 

  • Rattaya S, Benjakul S, Prodpran T (2009) Properties of fish skin gelatin film incorporated with seaweed extract. J Food Eng 95:151–157

    Article  CAS  Google Scholar 

  • Rawdkuen S, Suthiluk P, Kamhangwong D, Benjakul S (2012) Mechanical, physico-chemical, and antimicrobial properties of gelatin-based film incorporated with catechin-lysozyme. Chem Cent J 6:1–10

    Article  Google Scholar 

  • Ray SS, Okamoto M (2003) Polymer/layered silicate nanocomposites: a review from preparation to processing. Prog Polym Sci 28:1539–1641

    Article  CAS  Google Scholar 

  • Rhim J (2007) Potential use of biopolymer-based nanocomposite films in food packaging applications. Food Sci Biotechnol 16:691–709

    CAS  Google Scholar 

  • Rhim J, Ng P (2007) Natural biopolymer-based nanocomposite films for packaging applications. Crit Rev Food Sci Nutr 47:411–433

    Article  CAS  Google Scholar 

  • Santoso J, Yoshie-stark Y, Suzuki T (2004) Antioxidant activity of methanol extracts from Indonesian seaweeds in an oil emulsion model. Fish Sci 70:183–188

    Article  CAS  Google Scholar 

  • Shiku Y, Hamaguchi PY, Benjakul S, Visessanguan W, Tanaka M (2004) Effect of surimi quality on properties of edible films based on Alaska Pollack. Food Chem 86:493–499

    Article  CAS  Google Scholar 

  • Slade L, Levine H (1991) Beyond water activity: recent advances based on an alternative approach to the assessment of food quality and safety. Crit Rev Food Sci Nutr 30:115–360

    Article  CAS  Google Scholar 

  • Slinkard K, Singleton VL (1977) Total phenol analysis: automation and comparison with manual methods. AM J Enol Vitic 28:49–55

    CAS  Google Scholar 

  • Steel RGD, Torrie JH (1980) Principles and procedures of statistics: a biometrical approach, 2nd edn. McGraw-Hill, New York, p 633

    Google Scholar 

  • Tharanathan RN (2003) Biodegradable films and composite coatings: past, present and future. Trends Food Sci Technol 14:71–78

    Article  CAS  Google Scholar 

  • Theng BKG (1979) Formation and properties of clay–polymer complexes. Elsevier Scientific Publishing Company, Amsterdam

    Google Scholar 

  • Vazquez-Torres H, Canche-Escamilla G, Cruz-Ramos CA (1992) Coconut husk lignin. I Extraction and characterisation. J Applied Polym Sci 45:633–644

    Article  CAS  Google Scholar 

  • Wu J, Chen S, Ge S, Miao J, Li J, Zhang Q (2013) Preparation, properties and antioxidant activity of an active film from silver carp (Hypophthalmichthys molitrix) skin gelatin incorporated with green tea extract. Food Hydrocoll 32:42–51

    Article  Google Scholar 

  • Wu J, Ge S, Liu H, Wang S, Chen S, Wang J, Li J, Zhang Q (2014) Properties and antimicrobial activity of silver carp (Hypophthalmichthys molitrix) skin gelatin-chitosan films incorporated with oregano essential oil for fish preservation. Food Pack Shelf Life 2:7–16

    Article  Google Scholar 

  • Xu Y, Ren X, Hanna M (2006) Chitosan/clay nanocomposite film preparation and characterization. J Appl Polym Sci 99:1684–1691

    Article  CAS  Google Scholar 

  • Zheng JP, Li P, Ma YL, Yao KD (2002) Gelatin/montmorillonite hybrid nanocomposite. 1. Preparation and properties. J Appl Polym Sci 86:1189–1194

    Article  CAS  Google Scholar 

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Acknowledgments

The authors would like to express their sincere thanks to Prince of Songkla University and the TRF Distinguished Research Professor Grant for financial support. The ICAR-International Fellowship from Indian Council of Agricultural Research (ICAR), New Delhi, India, for Muralidharan Nagarajan is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Muralidharan Nagarajan & Soottawat Benjakul

  2. Department of Material Product Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Thummanoon Prodpran & Ponusa Songtipya

Authors
  1. Muralidharan Nagarajan
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  2. Soottawat Benjakul
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  3. Thummanoon Prodpran
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  4. Ponusa Songtipya
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Correspondence to Soottawat Benjakul.

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Nagarajan, M., Benjakul, S., Prodpran, T. et al. Properties and characteristics of nanocomposite films from tilapia skin gelatin incorporated with ethanolic extract from coconut husk. J Food Sci Technol 52, 7669–7682 (2015). https://doi.org/10.1007/s13197-015-1905-1

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  • DOI: https://doi.org/10.1007/s13197-015-1905-1

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