Abstract
The supramolecular nano-assemblies formed by electrostatic interactions of two oppositely charged lipid and polymer have been made and used as nanocarriers for curcumin to address its bioavailability and solubility issues. These curcumin encapsulated nano-supramolecular assemblies were characterized with respect to their size (dynamic light scattering), morphology (TEM, SEM), zeta potential (Laser Doppler Velocimetry), encapsulation efficiency (EE), curcumin loading (CL) etc. Stability of the nano-assemblies was assessed at different storage times as a function of varying pH and temperature. The physicochemical characterization of nano-assemblies was performed using Fourier Transform Infra Red Spectroscopy (FT-IR) and Differential Scanning Calorimetry (DSC). The in-vitro antioxidant lipid peroxidation (TBARS), radical scavenging (DPPH, NO, H2O2, reducing power) activity assays of powdered curcumin and nano-encapsulated curcumin were performed. It was found that nano-encapsulated curcumin were roughly spherical in shape, presented high positive zeta potential (>30 mV), monodisperse (polydispersity index <0.3), amorphous in nature, stable in the pH range of 2–6 and have enhanced antioxidant potency in comparison to crystalline curcumin in aqueous media. In conclusion, the curcumin encapsulated nanocarriers system has great potential as functional food ingredient of natural origin.
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References
Ak T, Gulçin I (2008) Antioxidant and radical scavenging properties of curcumin. Chem Biol Interact 174:27–37
Anitha A, Deepagan VG, Divya Rani VV et al (2011) Preparation, characterization, in vitro drug release and biological studies of curcumin loaded dextran sulphate-chitosan nanoparticles. Carbohydr Polym 84:1158–1164
Anon (1962) pH values of food products. Food Eng 34:98–99
Barthelemy S, Vergnes L, Moynier M et al (1998) Curcumin and curcumin derivatives inhibit Tat-mediated transactivation of type 1 human immunodeficiency virus long terminal repeat. Res Virol 149:43–52
Borra SK, Gurumurthy P, Mahendra J (2013) Antioxidant and free radical scavenging activity of curcumin determined by using different in vitro and ex vivo models. J Med Plant Res 7:2680–2690. doi:10.5897/JMPR2013.5094
Brannan RG, Connolly BJ, Decker EA (2001) Peroxynitrite: a potential initiator of lipid oxidation in food. Trends Food Sci Technol 12:164–173
Brugnerotto J, Lizardi J, Goycoolea FM et al (2001) An infrared investigation in relation with chitin and chitosan characterization. Polymer 42:3569–3580
Bursal E, Köksal E, Gulçin I et al (2013) Antioxidant activity and polyphenol content of cherry stem (Cerasus avium L.) determined by LC-MS/MS. Food Res Int 51:66–74
Champer J, Patel J, Fernando N et al (2013) Chitosan against cutaneous pathogens. AMB Express 3:37. doi:10.1186/2191-0855-3-37
Chattopadhyay I, Biswas K, Bandyopadhyay U et al (2004) Turmeric and curcumin: biological actions and medicinal applications. Curr Sci 87:44–53
Chien PJ, Sheu F, Huang WT, Su MS (2007) Effect of mol weight of chitosans on their antioxidative activities in apple juice. Food Chem 102:1192–1198
Delgado-vargas F, Paredes-López O (2003) Natural colorants for food and nutraceutical uses. CRC press LLC
Duclairoir C, Orecchioni AM, Depraetere P et al (2002) α-Tocopherol encapsulation and in vitro release from wheat gliadin nanoparticles. J Microencapsul 19:53–60
Dutta P, Dutta J, Tripathi V (2004) Chitin and chitosan: chemistry, properties and applications. J Sci Ind Res 63:20–31. doi:10.1002/chin.200727270
Gulcin I (2011) Antioxidant activity of food constituents-an overview. Arch Toxicol 86:345–396
Gulcin I, Elmasta M, Aboul-Enein HY et al (2012) Antioxidant activity of clove oil-A powerful antioxidant source. Arab J Chem 5:489–499
Gunes H, Gulen D, Mutlu R et al (2013) Antibacterial effects of curcumin: an in vitro minimum inhibitory concentration study. Toxicol Ind Health. doi:10.1177/0748233713498458
Hancock BC, Zografi G (1997) Characteristics and significance of the amorphous state in pharmaceutical systems. J Pharm Sci 86:1–12
Hellhammer J, Fries E, Buss C et al (2004) Effects of soy lecithin phosphatidic acid and phosphatidylserine complex (PAS) on the endocrine and psychological responses to mental stress. Stress (Amsterdam, Netherlands) 7:119–126. doi:10.1080/10253890410001728379
Hsieh RJ, Kinsella JE (1989) Oxidation of polyunsaturated fatty acids: mechanisms, products, and inhibition with emphasis on fish. Adv Food Nutr Res 33:233–341
Kanner J, German JB, Kinsella JE (1987) Initiation of lipid peroxidation in biological systems. Crit Rev Food Sci Nutr 25:317–364. doi:10.1080/10408398709527457
Kant V, Gopal A, Pathak NN et al (2014) Antioxidant and anti-inflammatory potential of curcumin accelerated the cutaneous wound healing in streptozotocin-induced diabetic rats. Int Immunopharmacol 20:322–30. doi:10.1016/j.intimp.2014.03.009
Ketron AC, Osheroff N (2014) Phytochemicals as anticancer and chemopreventive topoisomerase II poisons. Phytochem Rev 13:19–35
Khalil FA, Ali NH (2011) Protective effect of dietary antioxidants curcumin, vitamin C and Ginko biloba on oxidative stress in colonic rats induced by butylated hydroxyanisol. Aust J Basic Appl Sci 5:1489–1495
Kumar M, Ahuja M, Sharma SK (2008) Hepatoprotective study of curcumin-soya lecithin complex. Sci Pharm 76:761–774
Kumari A, Yadav SK, Pakade YB et al (2010) Development of biodegradable nanoparticles for delivery of quercetin. Colloids Surf B: Biointerfaces 80:184–192
Kunchandy E, Rao MNA (1990) Oxygen radical scavenging activity of curcumin. Int J Pharm 58:237–240
Lazar AN, Mourtas S, Youssef I et al (2013) Curcumin-conjugated nanoliposomes with high affinity for Aβ deposits: possible applications to alzheimer disease. Nanomedicine: Nanotechnol, Biol, Med 9:712–721
Lee DS, Je JY (2013) Gallic acid-grafted-chitosan inhibits foodborne pathogens by a membrane damage mechanism. J Agric Food Chem 61:6574–9. doi:10.1021/jf401254g
Love JD, Pearson a M (1974) Metmyoglobin and nonheme iron as prooxidants in cooked meat. J Agric Food Chem 22:1032–4
Mangolim CS, Moriwaki C, Nogueira AC et al (2014) Curcumin–β-cyclodextrin inclusion complex: stability, solubility, characterisation by FT-IR, FT-Raman, X-ray diffraction and photoacoustic spectroscopy, and food application. Food Chem 153:361–370
Mastellone I, Polichetti E, Grès S et al (2000) Dietary soybean phosphatidylcholines lower lipidemia: mechanisms at the levels of intestine, endothelial cell, and hepato-biliary axis. J Nutri Biochem 11:461–466
Miranda DTSZ, Batista VG, Grando FCC et al (2008) Soy lecithin supplementation alters macrophage phagocytosis and lymphocyte response to concanavalin A: a study in alloxan-induced diabetic rats. Cell Biochem Funct 26:859–865
Moncada S, Palmer RM, Higgs EA (1991) Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol Rev 43:109–142
Ogawa S, Decker EA, McClements DJ (2004) Production and characterization of O/W emulsions containing droplets stabilized by lecithin-chitosan-pectin multilayered membranes. J Agric Food Chem 52:3595–3600
Oke M, Jacob JK, Paliyath G (2010) Effect of soy lecithin in enhancing fruit juice/sauce quality. Food Res Int 43:232–240
Oyaizu M (1986) Studies on product of browning reaction prepared from glucosamine. Jpn J Nutr 44:778–84
Park Y, Kim M-H, Park S-C et al (2008) Investigation of the antifungal activity and mechanism of action of LMWS-chitosan. J Microbiol Biotechnol 18:1729–1734
Pathak L, Agrawal Y, Dhir A (2013) Natural polyphenols in the management of major depression. Expert Opin Invest Drugs 22:863–80. doi:10.1517/13543784.2013.794783
Ruch RJ, Cheng SJ, Klaunig JE (1989) Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 10:1003–1008
Sadzuka Y, Nagamine M, Toyooka T et al (2012) Beneficial effects of curcumin on antitumor activity and adverse reactions of doxorubicin. Int J Pharm 432:42–49
Santini A, Romano R, Meca G et al (2014) Antioxidant activity and quality of apple juices and puree after in vitro digestion. J Food Res 3:41–50
Schaffazick SR, Pohlmann AR, de Cordova CA et al (2005) Protective properties of melatonin-loaded nanoparticles against lipid peroxidation. Int J Pharm 289:209–213
Shahidi F (1997) Natural antioxidants: chemistry, health effects and applications. American Oil Chemists’ Society Press, Champaign
Sharma M (2012) Lipidome analysis reveals antifungal polyphenol curcumin affects membrane lipid homeostasis. Frontiers in Bioscience E4:1195. doi: 10.2741/E451
Singh N, Khullar N, Kakkar V, Kaur IP (2014) Attenuation of carbon tetrachloride-induced hepatic injury with curcumin-loaded solid lipid nanoparticles. BioDrugs: Clin Immunotherapeutics, Biopharmaceuticals Gene Therapy 28:297–312. doi:10.1007/s40259-014-0086-1
Sonvico F, Cagnani A, Rossi A et al (2006) Formation of self-organized nanoparticles by lecithin/chitosan ionic interaction. Int J Pharm 324:67–73
Sreejayan, Rao MN (1997) Nitric oxide scavenging by curcuminoids. J Pharm Pharmacol 49:105–107. doi:10.1111/j.2042-7158.1997.tb06761.x
St Angelo AJ (1996) Lipid oxidation on foods. Crit Rev Food Sci Nutr 36:175–224. doi:10.1080/10408399609527723
Stoilova I, Gargova S, Stoyanova A, Ho L (2005) Antimicrobial and antioxidant activity of the polyphenol mangiferin. Herba polonica 51:37–43
Tarladgis BG, Watts BM, Younathan MT, Dugan L (1960) A distillation method for the quantitative determination of malonaldehyde in rancid foods. J Am Oil Chem Soc 37:44–48
Tiwari SK, Agarwal S, Seth B et al (2014) Curcumin-loaded nanoparticles potently induce adult neurogenesis and reverse cognitive deficits in alzheimer’s disease model via canonical Wnt/β-catenin pathway. ACS Nano 8:76–103
Tonnesen HH, Karlsen J (1985) Studies on curcumin and curcuminoids. Z Lebensm Unters Forsch 180:402404
Ueda Y, Wang M-F, Irei AV et al (2011) Effect of dietary lipids on longevity and memory in the SAMP8 mice. J Nutr Sci Vitaminol 57:36–41
van Bracht E, Versteegden LRM, Stolle S, Verdurmen WPR, Woestenenk R, Raavé R, Hafmans T, Oosterwijk E, Brock R, van Kuppevelt TH, Daamen WF (2014) Enhanced cellular uptake of albumin-based lyophilisomes when functionalized with cell-penetrating peptide TAT in HeLa Cells. PLoS One 9(11):e110813
Vasilatos GC, Savvaidis IN (2013) Chitosan or rosemary oil treatments, singly or combined to increase turkey meat shelf-life. Int J Food Microbiol 166:54–58
Xie W, Xu P, Liu Q (2001) Antioxidant activity of water-soluble chitosan derivatives. Bioorganic Med Chem Letters 11:1699–1701
Yao H, Xu W, Shi X, Zhang Z (2011) Dietary flavonoids as cancer prevention agents. J Environ Sci Health Part C, Environ Carcinog Ecotoxicol Rev 29:1–31. doi:10.1080/10590501.2011.551317
Yen MT, Yang JH, Mau JL (2008) Antioxidant properties of chitosan from crab shells. Carbohydr Polym 74:840–844
Yen FL, Wu TH, Zeng CW T, Lin LT, Lin CC (2010) Curcumin nanoparticles improve the physicochemical properties of curcumin and effectively enhance its antioxidant and antihepatoma activities. J Agric Food Chem 58:7376–7382
Zhang D-W, Fu M, Gao S-H, Liu JL (2013) Curcumin and Diabetes: A Systematic Review. Evidence-based complementary and alternative medicine: eCAM 2013:636053. doi: 10.1155/2013/636053
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LPP and YKA would like to acknowledge Gujarat council on science and technology (GUJCOST) for research grant to carry out this work.
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Pathak, L., Kanwal, A. & Agrawal, Y. Curcumin loaded self assembled lipid-biopolymer nanoparticles for functional food applications. J Food Sci Technol 52, 6143–6156 (2015). https://doi.org/10.1007/s13197-015-1742-2
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DOI: https://doi.org/10.1007/s13197-015-1742-2