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Microbial based assay for specific detection of β-lactam group of antibiotics in milk

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Abstract

The spore forming Bacillus cereus (66) was screened for the induction of β-lactamase in presence of an inducer using iodometric assay. A significant induction in marker enzyme was observed in B. cereus 66 at maximum residual limit (MRL) of penicillin, ampicillin, cloxacillin, amoxicillin, cefalexin, and cephazolin belonging to β-lactam group of antibiotics. A microbial based assay, where enzyme induction was optimized at pH 7.0, temperature 30°C, and whey powder (0.25%) after 4 h of incubation. The spore based assay was tested with milk samples spiked with 6 different β-lactam antibiotics. The results were 100 and 83.33% in correlation with microbial receptor and inhibition based assay, respectively. Overall, spore based assay can be a useful and cost effective tool for the specific detection of β-lactam group of antibiotics in milk.

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Authors and Affiliations

  1. Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001, Haryana, India

    Sougata Das, Naresh Kumar, Raghu Hirikyathanahalli Vishweswaraiah, Manju Gaare, Vinai Kumar Singh & Anil Kumar Puniya

  2. The ICFAI University, Tripura, Kamalghat Sadar, Agarthala, 799210, West Tripura, India

    Lopamudra Haldar

Authors
  1. Sougata Das
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  2. Naresh Kumar
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  3. Raghu Hirikyathanahalli Vishweswaraiah
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  4. Lopamudra Haldar
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  5. Manju Gaare
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  6. Vinai Kumar Singh
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  7. Anil Kumar Puniya
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Correspondence to Naresh Kumar.

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Das, S., Kumar, N., Vishweswaraiah, R.H. et al. Microbial based assay for specific detection of β-lactam group of antibiotics in milk. J Food Sci Technol 51, 1161–1166 (2014). https://doi.org/10.1007/s13197-011-0609-4

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  • DOI: https://doi.org/10.1007/s13197-011-0609-4

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