Abstract
Compensatory base changes (CBCs) in helix II of rDNA ITS2, suggested as a molecular classifier for fungi, were analyzed in Mucor circinelloides and its varieties. Only a few CBCs were found in the complex. Three out of the four accepted formae (f. circinelloides, f. lusitanicus, f. janssenii) did not exhibit CBCs. One CBC was found between strains that form zygospores; consequently, CBC is not always concordant with mating experiments. Strains with two CBC are unable to breed. It is suggested that some strains of the M. circinelloides complex are at the beginning of speciation.
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The study was supported by Polish Ministry of Science and Higher Education grant NN_303 548 839. We wish to thank the reviewers for critically reviewing the manuscript and for their valuable comments.
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List of GenBank numbers and strains assigned to species and forma (where possible) level used in this study (DOC 100 kb)
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Pawłowska, J., Walther, G., Wilk, M. et al. The use of compensatory base change analysis of ITS2 as a tool in the phylogeny of Mucorales, illustrated by the Mucor circinelloides complex. Org Divers Evol 13, 497–502 (2013). https://doi.org/10.1007/s13127-013-0139-1
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DOI: https://doi.org/10.1007/s13127-013-0139-1