Abstract
Food allergy is a pathological condition that can lead to hives, swelling, gastrointestinal distress, cardiovascular and respiratory compromise, and even anaphylaxis. The lack of treatment resources emphasizes the necessity for new therapeutic strategies, and in this way, probiotics has been pointed out as an alternative, especially because of its immunomodulatory properties. The goal of this study was to evaluate the probiotic effect of Bifidobacterium longum subsp. longum 51A (BL51A) in a murine model of ovalbumin (OVA) food allergy, as well as to investigate the effect of the dose and viability of the bacteria on the proposed model. For this purpose, the probiotic effect was assessed by clinical, immunological, and histological parameters in mice treated or not with the BL51A and sensitized or not with OVA. Oral administration of BL51A prevented weight loss and reduced serum levels of IgE anti-OVA and of sIgA in the intestinal fluid. Also, it reduced the intestinal permeability, proximal jejunum damage, recruitment of eosinophils and neutrophils, and levels of eotaxin-1, CXCL1/KC, IL4, IL5, IL6, IL13, and TNF. Furthermore, the treatment was able to increase the levels of IL10. Investigating different doses administered, the level of 108 CFU showed the best results in terms of protective effect. In addition, the administration of the inactivated bacteria did not present any beneficial effect. Results demonstrate that BL51A promotes a systemic immunomodulatory protective effect in a murine model of food allergy that depends on the dose and viability of the bacteria, suggesting its use as probiotic in such disease.
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Data Availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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This work was supported by grants from the National Council for Scientific and Technological Development (CNPq) and the Coordination for the Improvement of Higher Education Personnel (CAPES). SSS received a PhD fellowship from CAPES and VCM received a PhD fellowship from CNPq. VNC, GDC, JRN, DCC, and FSM are CNPq fellowship holders.
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SSS, DCC, and FSM conceived and designed the work. SSS collected the data, and DCR, LMT, and DCC helped with data collection. SSS, DCC, and FSM analyzed and interpreted the data. VNC, GDC, JRN, DCC, and FSM contributed data and reagents or analysis tools. VCM wrote the paper. SSS, DCC, JRN, and FSM critically revised the article. DCC and FSM co-supervised the work. All the authors approved the final version of the article.
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All animal procedures were carried out according to the standards of the Brazilian Society of Laboratory Animal Science/Brazilian College for Animal Experimentation (available at http://www.mctic.gov.br/concea). This work was approved by the Ethics Committee in Animal Experimentation of the Federal University of Minas Gerais (CEUA/UFMG, protocol # 268/2015).
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Santos, S.S., Miranda, V.C., Trindade, L.M. et al. Bifidobacterium longum subsp. longum 51A Attenuates Signs of Inflammation in a Murine Model of Food Allergy. Probiotics & Antimicro. Prot. 15, 63–73 (2023). https://doi.org/10.1007/s12602-021-09846-9
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DOI: https://doi.org/10.1007/s12602-021-09846-9