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In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

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Abstract

In vitro regeneration of pigeon pea through organogenesis and somatic embryogenesis was demonstrated with pigeon pea cv. JKR105. Embryonic axes explants of pigeon pea showed greater regeneration of shoot buds on 2.5 mg L−1 6-benzylaminopurine (BAP) in the medium, followed by further elongation at lower concentrations. Rooting of shoots was observed on half-strength Murashige and Skoog (MS) medium with 2 % sucrose and 0.5 mg L−1 3-indolebutyric acid (IBA). On the other hand, the regeneration of globular embryos from cotyledon explant was faster and greater with thidiazuron (TDZ) than BAP with sucrose as carbohydrate source. These globular embryos were maturated on MS medium with abscisic acid (ABA) and finally germinated on half-strength MS medium at lower concentrations of BAP. Comparison of regeneration pathways in pigeon pea cv. JKR105 showed that the turnover of successful establishment of plants achieved through organogenesis was more compared to somatic embryogenesis, despite the production of more embryos than shoot buds.

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Acknowledgements

Authors express their indebtedness to Shri PS Dravid (President, JK Agri Genetics Ltd., Hyderabad, India), Shri SK Gupta (COO, JK Agri Genetics Ltd., Hyderabad, India) for providing necessary facilities. The author GK is thankful to Dr BU Singh (Advisor, JK Agri Genetics Ltd., Hyderabad, India) for guidance, valuable suggestions, continuous encouragement, and help extended in the manuscript preparation; and Dr Manish K. Patel (Associate Breeder, JKAL, Hyderabad, India) for providing the seed of pigeon pea cv. JKR105.

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  1. College of Biotechnology & Allied Sciences, Allahabad Agricultural Institute Deemed University, Allahabad, 211 007, UP, India

    Gaurav Krishna & Pramod W. Ramteke

  2. Biotechnology Division, JK Agri Genetics Ltd., 1-10-77, 4th Floor, Varun Towers, Begumpet, Hyderabad, 500 016, AP, India

    Gaurav Krishna, P. Sairam Reddy, Pogiri Rambabu, Sayed S. Sohrab, Debashis Rana & Parthasarathi Bhattacharya

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  1. Gaurav Krishna
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Krishna, G., Reddy, P.S., Ramteke, P.W. et al. In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105. Physiol Mol Biol Plants 17, 375–385 (2011). https://doi.org/10.1007/s12298-011-0079-1

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