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Plant regeneration via somatic embryogenesis in pigeonpea (Cajanus cajan L. Millsp)

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Abstract

Efficient plant regeneration via somatic embryogenesis has been developed in pigeonpea. Cotyledon and leaf explants from 10-day-old seedlings produced embryogenic callus and somatic embryos when cultured on Murashige and Skoog (MS) medium supplemented with 10 µm thidiazuron (TDZ). Subsequent withdrawal of TDZ from the induction medium resulted in the maturation and growth of the embryos into plantlets on MS basal medium. The rooted plantlets were transferred and acclimatized on vermiculite where they showed normal morphological characters.

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Authors and Affiliations

  1. National Research Centre for Plant Biotechnology, Indian Agricultural Research Institute, New Delhi-110012, India, , , , , , IN

    K. Sreenivasu, P. Ananda Kumar & R. P. Sharma

  2. National Bureau of Plant Genetic Resources, New Delhi-110012, India Fax no.: +91-11-5766420 E-mail: anand@bic-iari.ren.nic.in, , , , , , IN

    S. K. Malik

Authors
  1. K. Sreenivasu
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  2. S. K. Malik
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  3. P. Ananda Kumar
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  4. R. P. Sharma
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Received: 23 December 1996 / Revision received: 22 July 1997 / Accepted: 2 August 1997

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Sreenivasu, K., Malik, S., Ananda Kumar, P. et al. Plant regeneration via somatic embryogenesis in pigeonpea (Cajanus cajan L. Millsp). Plant Cell Reports 17, 294–297 (1998). https://doi.org/10.1007/s002990050395

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  • DOI: https://doi.org/10.1007/s002990050395

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