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Genomic and physiological analysis of C50 carotenoid-producing novel Halorubrum ruber sp. nov.

  • Microbial Genetics, Genomics and Molecular Biology
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Abstract

A novel haloarchaeal species designated as MBLA0099T was isolated from seawater near Yeongheung Island. Cells were Gram-negative, non-motile, red-pigmented, and rod-shaped. They grew at 10–45°C, within pH 5.5–9.0, and between 7.5% and 30% NaCl concentrations. Cells were able to grow without Mg2+ and were lysed in distilled water. The size of the whole-genome and G + C content of DNA was 3.02 Mb and 68.9 mol%, respectively. Phylogenetic analysis shows that the strain MBLA0099T belongs to the genus Halorubrum. The average nucleotide and amino acid identity, and in silico DNA-DNA hybridization values were below the species delineation threshold. Pan-genomic analysis revealed that 3.2% of all genes present in strain MBLA0099T were unique to the strain. The red carotenoid produced by strain MBLA0099T was subjected to spectrometric and chromatographic analyses and confirmed to be bacterioruberin as C50 carotenoid. Mevalonic acid, terpenoid backbone, and carotenoid biosynthesis pathway were annotated for strain MBLA0099T. The C50 carotenoid production by strain MBLA0099T was also enhanced under various stress conditions including relatively netural pH, high oxidative and salinity conditions. Additionally, the strain MBLA0099T-derived bacterioruberin showed the antioxidant activity with EC50 value of 12.29 µg/ml, based on the evaluation of DPPH free radical scavenging activity. The present study would be the first report on the identification of C50 carotenoid from the strain MBLA0099T representing a novel species of the genus Halorubrum, for which the name Halorubrum ruber sp. nov. is proposed. The typestrain used was MBLA0099T (= KCTC 4296T = JCM 34701T).

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Acknowledgements

This work was supported by Incheon National University Research Grant in 2019.

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  1. These authors contributed equally to this work.

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  1. Department of Bioengineering and Nano-Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea

    Chi Young Hwang, Eui-Sang Cho, Won Jong Rhee, Eunjung Kim & Myung-Ji Seo

  2. Division of Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea

    Won Jong Rhee, Eunjung Kim & Myung-Ji Seo

  3. Research Center for Bio Materials & Process Development, Incheon National University, Incheon, 22012, Republic of Korea

    Won Jong Rhee, Eunjung Kim & Myung-Ji Seo

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Hwang, C.Y., Cho, ES., Rhee, W.J. et al. Genomic and physiological analysis of C50 carotenoid-producing novel Halorubrum ruber sp. nov.. J Microbiol. 60, 1007–1020 (2022). https://doi.org/10.1007/s12275-022-2173-1

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