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Jeotgalibacillus haloalkalitolerans sp. nov., a novel alkalitolerant and halotolerant bacterium, isolated from the confluence of the Fenhe River and the Yellow River

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Abstract

A Gram-stain positive, aerobic, alkalitolerant and halotolerant bacterium, designated HH7-29 T, was isolated from the confluence of the Fenhe River and the Yellow River in Shanxi Province, PR China. Growth occurred at pH 6.0–12.0 (optimum, pH 8.0–8.5) and 15–40℃ (optimum, 32℃) with 0.5–24% NaCl (optimum, 2–9%). The predominant fatty acids (> 10.0%) were iso-C15:0 and anteiso-C15:0. The major menaquinones were MK-7 and MK-8. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified phospholipids. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that strain HH7-29 T was a member of the genus Jeotgalibacillus, exhibiting high sequence similarity to the 16S rRNA gene sequences of Jeotgalibacillus alkaliphilus JC303T (98.4%), Jeotgalibacillus salarius ASL-1 T (98.1%) and Jeotgalibacillus alimentarius YKJ-13 T (98.1%). The genomic DNA G + C content was 43.0%. Gene annotation showed that strain HH7-29 T had lower protein isoelectric points (pIs) and possessed genes related to ion transport and organic osmoprotectant uptake, implying its potential tolerance to salt and alkali. The average nucleotide identity, digital DNA–DNA hybridization values, amino acid identity values, and percentage of conserved proteins values between strain HH7-29 T and its related species were 71.1–83.8%, 19.5–27.4%, 66.5–88.4% and 59.8–76.6%, respectively. Based on the analyses of phenotypic, chemotaxonomic, phylogenetic and genomic features, strain HH7-29 T represents a novel species of the genus Jeotgalibacillus, for which the name Jeotgalibacillus haloalkalitolerans sp. nov. is proposed. The type strain is HH7-29 T (= KCTC 43417 T = MCCC 1K07541T).

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Data availability

No datasets were generated or analysed during the current study.

Abbreviations

MCCC:

Marine culture collection of China

KCTC:

Korean collection for type cultures

MEGA:

Molecular evolutionary genetics analysis

OCs:

Orthologous clusters

ANI:

Average nucleotide identity

dDDH:

Digital DNA-DNA hybridization

AAI:

Amino acid identity

POCP:

Percentage of conserved proteins

TLC:

Thin-layer chromatography

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Funding

This work was funded by the National Natural Science Foundation of China (32300115), the Basic Research Program of Shanxi Province (20210302124004), the Scientific and Technological Innovation Programs of Shanxi Agricultural University (2020BQ39), the Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi Province (2021L134) and by the Excellent Talents Come to Shanxi to Reward Scientific Research Projects (SXYBKY2019025).

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Authors and Affiliations

  1. College of Life Sciences, Shanxi Agricultural University, Taigu, 030801, Jinzhong, People’s Republic of China

    Ya-Fei Zhang, Yun-Lin Peng, Yong-Hui Xiao, Bing Yu, Ming-Yue Cao, Zhi-Ling Zhang, Le-Qun Huang & Yi Li

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  1. Ya-Fei Zhang
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  8. Yi Li
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Contributions

YFZ and YLP designed the experiments and wrote the manuscript. YHX, BY and MYC performed the genome analysis. ZLZ and LQH revised the manuscript. YL supervised the experiments.

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Correspondence to Yi Li.

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The GenBank accession numbers for the 16S rRNA gene sequence and the genome sequence of strain HH7-29 T are OR856029 and JAXQNN000000000, respectively.

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Zhang, YF., Peng, YL., Xiao, YH. et al. Jeotgalibacillus haloalkalitolerans sp. nov., a novel alkalitolerant and halotolerant bacterium, isolated from the confluence of the Fenhe River and the Yellow River. Antonie van Leeuwenhoek 117, 74 (2024). https://doi.org/10.1007/s10482-024-01968-8

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