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Improved tolerance of recombinant Chlamydomonas rainhardtii with putative 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase from Pyropia yezoensis to nitrogen starvation

  • Microbial Genetics, Genomics and Molecular Biology
  • Published:
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Abstract

In a previous study, a putative 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase (ACMSD) was highly expressed in a mutant strain of Pyropia yezoensis, which exhibited an improved growth rate compared to its wild strain. To investigate the functional role of the putative ACMSD (Pyacmsd) of P. yezoensis, the putative Pyacmsd was cloned and expressed in Chlamydomonas reinhardtii. Recombinant C. reinhardtii cells with Pyacmsd (Cr_Pyacmsd) exhibited enhanced tolerance compared to control C. reinhardtii cells (Cr_control) under nitrogen starvation. Notably, Cr_Pyacmsd cells showed accumulation of lipids in nitrogen-enriched conditions. These results demonstrate the role of Pyacmsd in the generation of acetyl-coenzyme A. Thus, it can be used to enhance the production of biofuel using microalgae such as C. reinhardtii and increase the tolerance of other biological systems to nitrogen-deficient conditions.

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Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (NRF-2018R1D1A1B07049359), Golden Seed Project Grant funded by the Ministry of Oceans and Fisheries (213008-05-4-SB910), and the research program of Korea Atomic Energy Research Institute (KAERI).

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Authors and Affiliations

  1. Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju, 61186, Republic of Korea

    Seo-jeong Park & Jong-il Choi

  2. Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, 56212, Republic of Korea

    Joon Woo Ahn

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  1. Seo-jeong Park
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  2. Joon Woo Ahn
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  3. Jong-il Choi
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Correspondence to Jong-il Choi.

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The authors declare that they have no known competing financial interests or personal relationship that could have appeared to influence the work reported in this paper.

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Park, Sj., Ahn, J.W. & Choi, Ji. Improved tolerance of recombinant Chlamydomonas rainhardtii with putative 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase from Pyropia yezoensis to nitrogen starvation. J Microbiol. 60, 63–69 (2022). https://doi.org/10.1007/s12275-022-1491-7

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  • DOI: https://doi.org/10.1007/s12275-022-1491-7

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