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Construction of a Streptomyces lydicus A01 transformant with a chit42 gene from Trichoderma harzianum P1 and evaluation of its biocontrol activity against Botrytis cinerea

  • Microbial Genetics, Genomics and Molecular Biology
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Abstract

Streptomyces lydicus A01 and Trichoderma harzianum P1 are potential biocontrol agents of fungal diseases in plants. S. lydicus A01 produces natamycin to bind the ergosterol of the fungal cell membrane and inhibits the growth of Botrytis cinerea. T. harzianum P1, on the other hand, features high chitinase activity and decomposes the chitin in the cell wall of B. cinerea. To obtain the synergistic biocontrol effects of chitinase and natamycin on Botrytis cinerea, this study transformed the chit42 gene from T. harzianum P1 to S. lydicus A01. The conjugal transformant (CT) of S. lydicus A01 with the chit42 gene was detected using polymerase chain reaction (PCR). Associated chitinase activity and natamycin production were examined using the 3, 5-dinitrosalicylic acid (DNS) method and ultraviolet spectrophotometry, respectively. The S. lydicus A01-chit42 CT showed substantially higher chitinase activity and natamycin production than its wild type strain (WT). Consequently, the biocontrol effects of S. lydicus A01-chit42 CT on B. cinerea, including inhibition to spore germination and mycelial growth, were highly improved compared with those of the WT. Our research indicates that the biocontrol effect of Streptomyces can be highly improved by transforming the exogenous resistance gene, i.e. chit42 from Trichoderma, which not only enhances the production of antibiotics, but also provides a supplementary function by degrading the cell walls of the pathogens.

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Authors and Affiliations

  1. School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, P. R. China

    Qiong Wu, Yingying Li, Yaqian Li, Kehe Fu, Chuanjin Yu & Jie Chen

  2. School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200030, P. R. China

    Linquan Bai

  3. Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, P. R. China

    Weicheng Liu & Caige Lu

Authors
  1. Qiong Wu
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  2. Linquan Bai
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  3. Weicheng Liu
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  4. Yingying Li
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  5. Caige Lu
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  6. Yaqian Li
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  7. Kehe Fu
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  8. Chuanjin Yu
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  9. Jie Chen
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Correspondence to Jie Chen.

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Wu, Q., Bai, L., Liu, W. et al. Construction of a Streptomyces lydicus A01 transformant with a chit42 gene from Trichoderma harzianum P1 and evaluation of its biocontrol activity against Botrytis cinerea . J Microbiol. 51, 166–173 (2013). https://doi.org/10.1007/s12275-013-2321-8

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  • DOI: https://doi.org/10.1007/s12275-013-2321-8

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