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Free radical scavenging and antielastase activities of flavonoids from the fruits of Thuja orientalis

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Abstract

Bioassay-guided fractionation of the MeOH extract of Thuja orientalis fruits using a DPPH (2,2-diphenyl-1-picrylhydrazyl) assay led to the isolation of 9 flavonoids: cupressuflavone (1), amentoflavone (2), robustaflavone (3), afzelin (4), (+)-catechin (5), quercitrin (6), hypolaetin 7-O-β-xylopyranoside (7), isoquercitrin (8) and myricitrin (9). Their chemical structures were determined by spectroscopic analyses. The free radical scavenging and human neutrophil elastase (HNE) inhibitory activities were evaluated for the isolated compounds. By DPPH scavenging assay, compounds 5, 6, 7, 8 and 9 showed anti-oxidant activities with IC50 values of 28.66, 31.19, 18.30, 26.63 and 15.10 μM, respectively. By ABTS [2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] scavenging assay, these compounds also exhibited potent anti-oxidant activities with IC50 values of 6.77, 13.96, 6.97, 22.79 and 9.96 μM, respectively. Of note, compounds 1, 2 and 3 showed significant HNE inhibitory activities with IC50 values of 8.09, 1.27 and 1.33 μM, respectively.

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Authors and Affiliations

  1. Functional Metabolite Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea

    Guang-Hua Xu, In-Ja Ryoo, Young-Hee Kim, Soo-Jin Choo & Ick-Dong Yoo

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  1. Guang-Hua Xu
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  2. In-Ja Ryoo
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  3. Young-Hee Kim
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  5. Ick-Dong Yoo
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Correspondence to Ick-Dong Yoo.

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Xu, GH., Ryoo, IJ., Kim, YH. et al. Free radical scavenging and antielastase activities of flavonoids from the fruits of Thuja orientalis . Arch. Pharm. Res. 32, 275–282 (2009). https://doi.org/10.1007/s12272-009-1233-y

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  • DOI: https://doi.org/10.1007/s12272-009-1233-y

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