Abstract
The present study focused to examine the phytochemical constituents and antioxidant properties of leaves, bark, and fruits of Ficus tsjahela Burm.f belonging to the well-known family Moraceae. The active principles were extracted by the method of soxhlation. The crude extracts were analyzed to establish a phytochemical profile using the standard principles, followed by the estimation of phenols, tannins, and flavonoid contents that were evaluated. The antioxidant effect was estimated by 1, 1-diphenyl-2-picryl hydroxyl (DPPH), nitric oxide, and metal chelating activity. Result data was analyzed by SPSS software (SPSS v.20), and Duncan test was used to find out statistical significance. The overall results revealed that methanol extract of F. tsjahela has showed the highest presence of bioactive compounds with comparative amounts of phenol, tannins, and flavonoid contents. Ascorbic acid, BSA, rutin, gallic acid, and glucose were used as the reference standard. The IC50 (concentration required for 50% inhibition) was calculated for DPPH radical scavenging activity. The total phenol content was 187.3 ± 12.1 mg (GAE)/g, 949.7 ± 124.6 mg GAE/g, and 834.4 ± 30.7 mg GAE/g extract for the leaf, bark, and fruit, respectively, and the total flavonoid content was 15.20 ± 0.71 mg RE/g, 129.4 ± 11.08 mg RE/g, and 65.64 ± 6.80 mg RE/g extract for the leaf, bark, and fruit, respectively.
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We thankfully acknowledge the financial support from the council of DST-PURSE, New Delhi, and Dept. of Botany, Bharathiar University, for providing the essential things.
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Velmurugan, T., Sebasthi, A.D., Thangavel, S. (2023). Estimation of Primary and Secondary Metabolites and In Vitro Free Radical Scavenging Activities with Ficus tsjahela Burm.F. Crude Extracts. In: Arunachalam, K., Yang, X., Puthanpura Sasidharan, S. (eds) Natural Product Experiments in Drug Discovery. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2683-2_4
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DOI: https://doi.org/10.1007/978-1-0716-2683-2_4
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