Skip to main content
SpringerLink
Log in

Inhibitory effects of Siegesbeckiae herba extract on angiogenesis and adipogenesis

  • Research Paper
  • Published:
Biotechnology and Bioprocess Engineering Aims and scope Submit manuscript

Abstract

In this study, 18 natural products were screened via anti-angiogenesis experiments, and 1 candidate product was identified, Siegesbeckiae herba, which exerted dose-dependent inhibitory effects against angiogenesis, adipogenesis, and cell adhesion. S. herba extract (SHE) exhibits an angiogenesis inhibitory effect superior to that of the EGCG from green tea leaves. The expression level of angiogenesis and adipogenesis-related signal molecules in the western blotting was reduced by increasing the amount of added SHE. Moreover, a diet supplemented with SHE was deemed more effective in inducing weight loss in LB mice than a representative synthetic diet drug, orlistat, which incidently caused the side effect of denuding the mice of their hair. These results indicate that S. herba may prove to be a useful anti-adipogenic compound, and these in vitro results may be reflected later under in vivo conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Pataky, Z., E. Bobbioni-Harsch, and A. Golay (2009) Obesity: A complex growing challenge. Exp. Clin. Endocr. Diab. 118: 427–433.

    Article  Google Scholar 

  2. Frezza, E. E., M. S. Wachtel, and B. T. Ewing (2006) The impact of morbid obesity on the state economy: An initial evaluation. Surg. Obes. Relat. Dis. 2: 504–508.

    Article  Google Scholar 

  3. Folkman, J. and Y. Shing (1992) Angiogenesis. J. Biol. Chem. 267: 10931–10934.

    CAS  Google Scholar 

  4. Doldi, N., M. Bassan, M. Gulisano, V. Broccoli, E. Boncinelli, and A. Ferrari (1996) Vascular endothelial growth factor messenger ribonucleic acid expression in human ovarian and endometrial cancer. Gynecol. Endocrinol. 10: 375–382.

    Article  CAS  Google Scholar 

  5. Cao, Y. (2007) Angiogenesis modulates adipogenesis and obesity. J. Clin. Invest. 117: 2362–2368.

    Article  CAS  Google Scholar 

  6. Crandall, D. L., G. J. Hausman, and J. G. Kral (1997) A review of the microcirculation of adipose tissue: Anatomic, metabolic, and angiogenic perspectives. Microcirculation 4: 211–232.

    Article  CAS  Google Scholar 

  7. Hausman, G. J. and R. L. Richardson (2004) Adipose tissue angiogenesis. J. Anim. Sci. 82: 925–934.

    CAS  Google Scholar 

  8. Neels, J. G., T. Thinnes, and D. J. Loskutoff (2004) Angiogenesis in an in vivo model of adipose tissue development. FASEB J. 18: 983–985.

    CAS  Google Scholar 

  9. Voros, G., E. Maquoi, D. Demeulemeester, N. Clerx, D. Collen, and H. R. Lijnen (2005) Modulation of angiogenesis during adipose tissue development in murine models of obesity. Endocrinol. 146: 4545–4554.

    Article  CAS  Google Scholar 

  10. Christiaens, V. and H. R. Lijnen (2010) Angiogenesis and development of adipose tissue. Mol. Cell Endocrinol. 318: 2–9.

    Article  CAS  Google Scholar 

  11. Rudkowska, I., C. E. Roynette, I. Demonty, C. A. Vanstone, S. Jew, and P. J. Jones (2005) Diacylglycerol: Efficacy and mechanism of action of an anti-obesity agent. Obes. Res. 13: 1864–1876.

    Article  CAS  Google Scholar 

  12. Obeid, O. A., S. T. Bittar, N. Hwalla, and P. W. Emery (2005) Effect of diet supplementation with glutamine, dihydroxyacetone, and leucine on food intake, weight gain, and postprandial glycogen metabolism of rats. Nutrition 21: 224–229.

    Article  CAS  Google Scholar 

  13. Tyler, C., A. Prestwich, A. Ormond, and M. MacDougald (2007) Wnt/b-catenin signaling in adipogenesis and metabolism. Curr. Opin. Cell Biol. 19: 612–617.

    Article  Google Scholar 

  14. Yun, J. W. (2010) Possible anti-obesity therapeutics from nature — A review. Phytochem. 71: 1625–1641.

    Article  CAS  Google Scholar 

  15. Oh, S., K. S. Kim, Y. S. Chung, M. Shong, and S. B. Park (2009) Anti-obesity agents: A focused review on the structural classification of therapeutic entities. Curr. Top Med. Chem. 9: 466–481.

    Article  CAS  Google Scholar 

  16. Chou, G. X., R. M. Jin, Z. T. Wang, and C. X. Chen (2006) Study on antithrombotic fraction of Herba Siegesbeckiae. Zhong Yao Cai 29: 235–237.

    CAS  Google Scholar 

  17. Hu, H. H., L. X. Tang, and X. M. Li (2004) Experimental research of effect of crude and processed Herba Siegesbeckiae on anti-inflammation and anti-rheumatism. Zhongguo Zhong Yao Za Zhi 29: 542–545.

    Google Scholar 

  18. Kim, I. Y. (1980) A study on the diuretic action of Siegesbeckiae Herba ethanol extract in dogs. J. Korean Pharm. Sci. 10: 5–13.

    Google Scholar 

  19. Lee, I., N. S. Seong, and Y. J. Lee (2005) A study on the protective effects of Siegesbeckiae Herba on neurotoxicity induced by N-methyl-D-aspartic acid (NMDA). Kor. J. Herbology 20: 121–132.

    Google Scholar 

  20. Qian, R., C. Zhang, and H. Fu (2000) Study on therapeutic mechanism of anti-rheumatism action of herba siegesbeckiae. Zhongguo Zhong Xi Yi Jie He Za Zhi 20: 192–195.

    CAS  Google Scholar 

  21. Ramírez-Zacarías, J. L., Castro-Muñozledo, F., and W. Kuri-Harcuch (1992) Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochemistry. 97: 493–497.

    Article  Google Scholar 

  22. Lee, D. H., J. P. MacIntyre, E. Wang, D. J. Hudson, A. Ishaque, J. A. Conant, B. L. Pope, and C.Y. Lau (1994) A leukocyte lipid upregulates the avidity of lymphocyte function-associated antigen-1. Biochem. Bioph. Res. Comm. 199: 319–326.

    Article  CAS  Google Scholar 

  23. Vandermeeren, M., S. Janssens, M. Borgers, and J. Geysen (1997) Dimethylfumarate is an inhibitor of cytokine-induced E-selectin, VCAM-1, and ICAM-1 expression in human endothelial cells. Biochem. Bioph. Res. Comm. 234: 19–23.

    Article  CAS  Google Scholar 

  24. Carmelit, P., M. G. Lampugnani, L. Moons, F. Breviaro, V. Compernolle, F. Bono, G. Balconi, R. Spagnuolo, B. Oosthuyse, M. Dewerchin, A. Zanetti, and A. Angellilo (1999) Targeted deficiency or cystolic truncation of the VE-cadherin gene in mice impairs VEGF-mediated endothelial survival and angiogenesis. Cell 98: 147–157.

    Article  Google Scholar 

  25. Zhang, D., M. Bar-Eli, S. Meloche, and P. Brodt (2004) Dual regulation of MMP-2 expression by the type 1 insulin-like growth factor receptor: The phosphatidylinositol 3-kinase/Akt and Raf/ERK pathways transmit opposing signals. J. Biol. Chem. 279: 19683–19690.

    Article  CAS  Google Scholar 

  26. Ballinger, A. and S. R. Peikin (2002) Orlistat: Its current status as an anti-obesity drug. Eur. J. Pharmacol. 440: 109–117.

    Article  CAS  Google Scholar 

  27. Jandacek, R. J. and S. C. Woods (2004) Pharmaceutical approaches to the treatment of obesity. Drug Discov. Today 15: 874–880.

    Article  Google Scholar 

  28. Birari, R. B. and K. K. Bhutani (2007) Pancreatic lipase inhibitors from natural sources: Unexplored potential. Drug Discov. Toda. 12: 879–889.

    Article  CAS  Google Scholar 

  29. Weigle, D. S. (2003) Pharmacological therapy of obesity: Past, present, and future. J. Clin. Endocr. Metab. 88: 2462–2469.

    Article  CAS  Google Scholar 

  30. Choi, Y. S., H. Park, and S. Jeong (2006) Role of PI3-kinase/akt pathway in the activation of etoposide-induced NF-Kb transcription factor. J. Microbiol. Biotechn. 16: 391–398.

    CAS  Google Scholar 

  31. Tang, F. Y., N. Nhan, and M. Mohsen (2003) Green tea catechins inhibit VEGF-induced angiogenesis in vitro through suppression of VE-cadherin phosphorylation and inactivation of AKT molecule. Int. J. Cancer 106: 871–878.

    Article  CAS  Google Scholar 

  32. Tang, F. Y. and M. Meydani (2001) Green tea catechins and vitamin E inhibit angiogenesis of human microvascular endothelial cells through suppression of IL-8 production. Nutr. Cancer 41: 119–125.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Fisheries and Ocean Science, Chonnam National University, Yosu, 550-749, Korea

    Jae-Ho Hwang

  2. Department of Biotechnology, Chonnam National University, Yosu, 550-749, Korea

    Jong-Deog Kim

  3. Research Center on Anti-Obesity and Health Care, Chonnam National University, Yosu, 550-749, Korea

    Jong-Deog Kim

Authors
  1. Jae-Ho Hwang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jong-Deog Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jong-Deog Kim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hwang, JH., Kim, JD. Inhibitory effects of Siegesbeckiae herba extract on angiogenesis and adipogenesis. Biotechnol Bioproc E 16, 144–152 (2011). https://doi.org/10.1007/s12257-011-0012-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12257-011-0012-z

Keywords

Search

Navigation