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CRP and LOX-1: a Mechanism for Increasing the Tumorigenic Potential of Colorectal Cancer Carcinoma Cell Line

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Pathology & Oncology Research

Abstract

Chronic inflammation and dyslipidemia are associated with an increase in the incidence of colorectal cancer (CRC). Serum C- reactive protein (CRP) and oxidized low-density lipoprotein (oxLDL), as Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) ligands, increase during inflammation and dyslipidemia, respectively. To evaluate the effects of CRP on the expression of important genes involved in the development of CRC, the CRC cell line, LS174T, was treated with the commercial CRP. Based on the Real-time PCR data, in the presence of CRP, LOX-1, CEA, MMP1, and MMP2 mRNA expression significantly increased, compared to the control group. Moreover, in the presence of CRP, secretion, and expression of CEA in the cell lysate and conditioned media increased in a concentration-dependent manner. The results of flow cytometry showed that expression of LOX-1 receptors at the cell surface increased significantly in the presence of 10 mg/L of CRP. However, inhibition of LOX-1 receptors with a specific monoclonal antibody reduced the effects of CRP on protein/mRNA expression. In conclusion, Increased CRP level, can potentially elevate the expression of important genes in CRC by stimulating LOX-1 receptors.

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Abbreviations

LOX-1 or OLR1:

Lectin-like oxidized low-density lipoprotein receptor-1

CEA :

Carcinoembryonic antigen

MMP :

Matrix Metalloproteinase

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Acknowledgments

The research presented in this article is part of the dissertation of Mousa Ghazi-Khanloosani, to receive a master’s degree in biochemistry. This project was sponsored by Semnan University of Medical Sciences under the project no. 892.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran

    Mousa Ghazi-Khanloosani, Ahmad Reza Bandegi & Abbas Pakdel

  2. Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan, Iran

    Ahmad Reza Bandegi & Abbas Pakdel

  3. Cancer Research Center and Department of Immunology, Semnan University of Medical Sciences, Semnan, Iran

    Parviz Kokhaei & Mehdi Barati

  4. Immune and Gene Therapy Lab, Cancer Centre Karolinska, Karolinska University Hospital, Stockholm, Sweden

    Parviz Kokhaei

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  1. Mousa Ghazi-Khanloosani
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Correspondence to Abbas Pakdel.

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The study was approved by the ethics Committee of Semnan University of Medical Sciences.

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Ghazi-Khanloosani, M., Bandegi, A.R., Kokhaei, P. et al. CRP and LOX-1: a Mechanism for Increasing the Tumorigenic Potential of Colorectal Cancer Carcinoma Cell Line. Pathol. Oncol. Res. 25, 1467–1475 (2019). https://doi.org/10.1007/s12253-018-0507-4

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