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Resistance to Ralstonia solanacearum of Sexual Hybrids Between Solanum commersonii and S. tuberosum

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Abstract

This research was carried out to study the levels of bacterial wilt resistance and genetic diversity of (near)pentaploid sexual hybrids between S. commersonii (2n = 2x = 24, 1EBN) and cultivated S. tuberosum. Following artificial inoculations with Ralstonia solanacearum, wilting degree was estimated on a scale from 0 to 4, and seven genotypes of 26 (27%) displaying a S. commersonii like behavior were identified. Latent bacterial colonizations were detected in roots of symptomless S. commersonii and hybrids, whereas no bacterial populations were detected within stems. This suggests that the movement and/or growth of the bacterium in the aerial part were strongly inhibited. A molecular study with AFLP markers clustered hybrids into nine groups and provided evidence that resistant hybrids were slightly more similar to cultivated S. tuberosum than to the wild parent. This is important in view of the re-establishment of the cultivated genetic background through backcrosses. Hybrids displayed good fertility and are being used for further breeding efforts.

Resumen

Esta investigación fue realizada para estudiar los niveles de resistencia a la marchitez bacteriana y la diversidad genética de híbridos sexuales (casi) pentaploides entre S. commersoni y S. tuberosum cultivado. Después de las inoculaciones artificiales con Ralstonia solanacearum, la marchitez fue estimada en una escala de 0 a 4 y se identificaron siete genotipos de 26 (27%) que se comportaron como S. commersoni. Se detectaron colonizaciones bacterianas latentes en raíces que no presentaban síntomas de S. commersoni e híbridos mientras que no se detectó población bacteriana dentro de los tallos. Esto sugiere que el movimiento y/o crecimiento de la bacteria en la parte aérea fue fuertemente inhibido. Un estudio molecular con marcadores AFLP agrupó los híbridos en nueve grupos y dio la evidencia de que los híbridos fueron ligeramente más similares al S. tuberosum cultivado que sus progenitores silvestres. Esto es importante en vista del restablecimiento de los antecedentes genéticos de la especie cultivada por medio de retro cruzamientos. Los híbridos mostraron buena fertilidad y están siendo usados para mejoramiento.

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Acknowledgments

This work is dedicated to the fondest memory of Stan Peloquin, mentor and great friend for our group. We gratefully acknowledge the technical support of S. Alberino, I. Caruso and R. Garramone. This is contribution no. 191 from the Dept. of Soil, Plant, Environmental and Animal Production Sciences. The experiments comply with the current Italian laws.

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Authors and Affiliations

  1. Department Soil, Plant, Environmental and Animal Production Sciences, University of Naples Federico II, Via Università 100, 80055, Portici, Italy

    Domenico Carputo, Riccardo Aversano, Amalia Barone, Antonio Di Matteo, Massimo Iorizzo & Luigi Frusciante

  2. Ente Nazionale Sementi Elette (ENSE) Sezione di Battipaglia, Strada Statale 18, Km 77,700, 84091, Battipaglia, Italy

    Loredana Sigillo

  3. Department Horticulture, Botany and Plant Pathology, 1University of Naples Federico II, Via Università 100, 80055, Portici, Italy

    Astolfo Zoina

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  1. Domenico Carputo
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  2. Riccardo Aversano
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  3. Amalia Barone
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  4. Antonio Di Matteo
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  6. Loredana Sigillo
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  7. Astolfo Zoina
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  8. Luigi Frusciante
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Correspondence to Domenico Carputo.

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Carputo, D., Aversano, R., Barone, A. et al. Resistance to Ralstonia solanacearum of Sexual Hybrids Between Solanum commersonii and S. tuberosum . Am. J. Pot Res 86, 196–202 (2009). https://doi.org/10.1007/s12230-009-9072-4

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  • DOI: https://doi.org/10.1007/s12230-009-9072-4

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