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Multiplication ofPseudomonas solanacearum in resistant potato plants and the establishment of latent infections

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Abstract

When 1-mo-old plants of a wilt-resistant clone ofSolanum phureja (1386.15) were stem-inoculated with three strains ofPseudomonas solanacearum (K60, S123, and S206), the bacteria multiplied rapidly at the point of inoculation and then moved in the vascular system to other parts of the stem. Resistant plants showed a remarkable ability to support relatively high populations of the bacterium in the absence of disease symptoms. Although multiplication in this resistant clone was substantially less than in susceptible Russet Burbank potato plants, large numbers of bacteria (up to 624 × 104 cells of K60 per 5-cm stem segment) reached the base of the stem of plants maintained at high temperature (28°C) for 20 days after stem inoculation. From the base of the stem, the bacteria moved rapidly into the roots and tubers. Strains ofP. solanacearum differed in their ability to cause latent tuber infection in different resistant potato clones. When 11S.phureja ×S. tuberosum hybrids were stem-inoculated, maintained at 28°C for 3 wk and then grown to maturity at 20°C., most of the clones yielded tubers infected by one or more strains. The race 1 strain (K60) was the most infectious; 53.8% of all tubers harvested from all plants inoculated with this isolate carried latent infections. Because one clone (BR 53.1) never yielded infected tubers, there appear to be genetic factors which may be useful in breeding programs aimed at eliminating latent tuber infection.

Resumen

Cuando se inocularon plantas de 1 mes de edad del clon deSolanum phureja, 1886.15 (resistente) con tres cepas dePseudomonas solanacearum (K60, S123 y S206) las bacterias se multiplicacion rápidamente en el lugar de la inoculación y se esparcieron en el tallo por medio de los haces vasculares. Aunque la multiplicación en este clon resistente fué mucho menor que en una planta de papa susceptible, como Russet Burbank, la población de bacterias alcanzó 624 × 104 células por segmento de 5 cm de largo de tallo en la base de plantas mantenidas a una temperatura alta (28°) por 20 días. De la base del tallo, la bacteria se esparció rápidamente a las raíces y tubérculos. Diferentes cepasde P. solanacearum difirieron en su abilidad de causar infecciones latentes en los tubérculos de clones resistentes. Cuando se inocularon 11 híbridos deS. phureja × S. tuberosum en los tallos y las plantas se mantuvieron a 28°C por 3 semanas y luego se dejaron madurar a 20°C., casi todos los clones produjeron tubérculos infectados por una o más cepas. La cepa de raza 1, K60 fué la más infecciosa; 53.8% de todos los tubérculos producidos por todas las plantas inoculadas resultaron infectados por esta bacteria. Como uno de los clones resistentes (BR 53.1) nunca produjo tubérculos infectados, se sugiere que esta es una característica genética que puede ser muy útil en programas de mejoramiento en que se intente eliminar el problema de infecciones latentes en los tubérculos.

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Authors and Affiliations

  1. Department of Plant Pathology, University of Wisconsin-Madison, 53706, Madison, WI

    Luigi Ciampi & Luis Sequeira

  2. Institute de Ecología y Evolución, Universidad Austral de Chile, Casilla 567, Valdivia, Chile

    Luigi Ciampi

Authors
  1. Luigi Ciampi
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  2. Luis Sequeira
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Additional information

Research supported by the College of Agricultural and Life Sciences, University of Wisconsin-Madison (Project 1481) and by a grant from the International Potato Center, Lima, Peru.

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Ciampi, L., Sequeira, L. Multiplication ofPseudomonas solanacearum in resistant potato plants and the establishment of latent infections. American Potato Journal 57, 319–329 (1980). https://doi.org/10.1007/BF02854027

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  • DOI: https://doi.org/10.1007/BF02854027

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