Abstract
Culturable psychrophilic prokaryotes were obtained of samples of glacier sediment, seaside mud, glacier melted ice, and Deschampsia antarctica rhizosphere from Collins glacier, Antarctica. The taxonomic classification was done by a culture-dependent molecular approach involving the Amplified Ribosomal DNA Restriction Analysis. Two hundred sixty colonies were successfully isolated and sub-cultivated under laboratory conditions. The analysis showed a bacterial profile dominated by Beta-proteobacteria (35.2%) followed by Gamma-proteobacteria (18.5%), Alpha-proteobacteria (16.6%), Gram-positive with high GC content (13%), Cytophaga–Flavobacterium–Bacteroides (13%) and Gram-positive with low GC content (3.7%). Eleven of the isolates have been reported previously and the others microorganisms remain uncharacterized. The isolated microorganisms here could be a potential source for biotechnological products, such as cold-active enzymes and secondary metabolites.
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The authors are grateful to Leandro G. Ordoñez for his technical support. This work was partially financed by grant Antarctica Des S-3607, DIUFRO D109-0081, D108-2002, FONDEF Grant D03I-1079. Sergio A. Garcia thanks CONACyT for his scholarship 209658. We thank the INACH for its logistic support during the stay at the “Prof. Julio Escudero” Scientific Base. We thank B. Yehezkel for critical reading of the manuscript.
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García-Echauri, S.A., Gidekel, M., Gutiérrez-Moraga, A. et al. Isolation and phylogenetic classification of culturable psychrophilic prokaryotes from the Collins glacier in the Antarctica. Folia Microbiol 56, 209–214 (2011). https://doi.org/10.1007/s12223-011-0038-9
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DOI: https://doi.org/10.1007/s12223-011-0038-9