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Isolation and Identification of Bacteria from an Ice Wedge of the Mamontova Gora Glacial Complex (Central Yakutia)

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Abstract—

Analysis of the aerobic heterotrophic component of bacterial communities from samples of poorly studied ancient ice wedge from the Mamontova Gora section (Central Yakutia), was carried out. The abundance of colony forming cells (CFU) of aerobic heterotrophic bacteria detected by plating of the thawed samples onto an agar nutrient media varied within 103‒104 CFU/mL. At the same time, the total number of intact cells was only 1−2 orders of magnitude higher. The taxonomic position of bacteria was determined by analysis of the 16S rRNA gene sequences. These bacteria were represented by three phyla: Actinobacteria, Firmicutes, and Proteobacteria. The temperature growth range of 8–20°C, which is characteristic of psychrotolerant bacteria, proved to be a specific physiological feature of the isolated strains. Most bacteria were found to be sensitive to a wide range of antibiotics.

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Funding

This study was supported in part by the Russian Foundation for Basic Research, project no. 16-05-00296. The work on the isolation of microorganisms was supported by FASO as part of a government task (Collection of Unique and Extremophilic Microorganisms for Biotechnological Purposes) for fundamental research at the Research Center of Biotechnology (Russian Academy of Sciences).

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 119071, Moscow, Russia

    S. N. Filippova, N. A. Surgucheva, A. L. Mulyukin & V. F. Gal’chenko

  2. Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, 119071 , Moscow, Russia

    T. V. Kolganova

  3. Department of Geology, Moscow State University, 119991, Moscow, Russia

    M. Yu. Cherbunina & A. V. Brushkov

Authors
  1. S. N. Filippova
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  2. N. A. Surgucheva
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  3. T. V. Kolganova
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  4. M. Yu. Cherbunina
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  5. A. V. Brushkov
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  6. A. L. Mulyukin
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  7. V. F. Gal’chenko
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Correspondence to S. N. Filippova.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by A. Panyushkina

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Filippova, S.N., Surgucheva, N.A., Kolganova, T.V. et al. Isolation and Identification of Bacteria from an Ice Wedge of the Mamontova Gora Glacial Complex (Central Yakutia). Biol Bull Russ Acad Sci 46, 234–241 (2019). https://doi.org/10.1134/S1062359019030026

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  • DOI: https://doi.org/10.1134/S1062359019030026

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