Abstract
Supplementation of the rumen ciliate Diploplastron affine growth medium with commercial chitin stimulated growth of ciliates and the density of their population was positively correlated with chitin doses (r = 0.95; p < 0.01). The cell-free extracts prepared from bacteria-free ciliates degraded chitin to N-acetyl-d-glucosamine and chitobiose. Three exochitinases, two endochitinases and two β-N-acetylglucosaminidases were identified in the cell-free extract of protozoa. The molar mass of exochitinases was 80, 65 and 30 kDa, and endochitinases 75 and 50 kDa; the molar mass of one of the identified β-N-acetylglucosaminidases was 45 kDa.
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Bełżecki, G., Miltko, R., Michałowski, T. et al. Chitinolytic activity of the sheep rumen ciliate Diploplastron affine . Folia Microbiol 53, 201–203 (2008). https://doi.org/10.1007/s12223-008-0025-y
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DOI: https://doi.org/10.1007/s12223-008-0025-y