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Protective effects of selenium against zearalenone-induced apoptosis in chicken spleen lymphocyte via an endoplasmic reticulum stress signaling pathway

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Cell Stress and Chaperones Aims and scope

Abstract

Selenium (Se), an antioxidant agent, provides significant protection from reactive oxygen species (ROS)-induced cell damage in vivo and in vitro. However, it is unclear whether Se can protect against zearalenone (ZEN)-induced apoptosis in chicken spleen lymphocyte. In this study, we investigated the underlying mechanism of the apoptosis induced by ZEN in chicken spleen lymphocyte and further evaluated the protective mechanism of Se on ZEN-induced apoptosis. The results show that ZEN induced an increase in ROS generation and lipid peroxidation, and a decrease in levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and glutathione (GSH). The results of apoptosis morphologically from acridine orange/ethidium bromide (AO/EB) fluorescent staining and flow cytometry analysis show apparent apoptosis in the ZEN-treated group, and was confirmed by the upregulation of caspase-3, -12 and downregulation of Bcl-2. Meanwhile, ZEN activated the endoplasmic reticulum (ER) stress by upregulating ER stress-related molecular sensors (GRP78, ATF6, ATF4, IRE). However, co-treatment with Se effectively blocked ROS generation, improved antioxdative capacity, and reversed apoptosis and ER stress-related genes and protein expression. Taken together, these data suggest that oxidative stress and ER stress play a vital role in ZEN-induced apoptosis, and Se had a significant preventive effect on ZEN-induced apoptosis in chicken spleen lymphocyte via ameliorating the ER stress signaling pathway.

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Abbreviations

ZEN:

Zearalenone

ROS:

Reactive oxygen species

ER:

Endoplasmic reticulum

IRE:

Inositol requiring enzyme

PERK:

Protein kinase RNA (PKR)-like ER kinase

ATF 6:

Activating transcription factor 6

GRP78:

Glucose-regulated protein 78

ATF4:

Activating transcription factor 4

Se:

Selenium

SOD:

Superoxide dismutase

GSH-Px:

Glutathione peroxidase

GSH:

Glutathione

MDA:

Malondialdehyde

CAT:

Catalase

AO/EB:

Acridine orange/Ethidium bromide

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Funding

This work received financial support from the National Key Research and Development Program of China (No. 2017YFD0502200).

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Authors and Affiliations

  1. Department of Veterinary Obstetrics, College of Veterinary Medicine, Northeast Agricultural 8 University, Harbin, 150030, China

    Yinxia Xiao, Shuchen Zhao, Kexiang Liu, Zhanjun Lu & Zhenzhong Hou

  2. Department of Veterinary Internal Medicine, College of Veterinary Medicine, Northeast 11 Agricultural University, Harbin, 150030, China

    Shiwen Xu

  3. College of Veterinary Medicine, Northeast Agricultural University, Animal Hospital, NO.59 Wood Street, Xiangfang District, Harbin, 150030, China

    Zhenzhong Hou

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  1. Yinxia Xiao
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  2. Shiwen Xu
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  3. Shuchen Zhao
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Correspondence to Zhenzhong Hou.

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Xiao, Y., Xu, S., Zhao, S. et al. Protective effects of selenium against zearalenone-induced apoptosis in chicken spleen lymphocyte via an endoplasmic reticulum stress signaling pathway. Cell Stress and Chaperones 24, 77–89 (2019). https://doi.org/10.1007/s12192-018-0943-9

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  • DOI: https://doi.org/10.1007/s12192-018-0943-9

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