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Differential expression of myocardial heat shock proteins in rats acutely exposed to fluoride

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Cell Stress and Chaperones Aims and scope

Abstract

Acute fluoride (F−) toxicity is known to cause severe cardiac complications and leads to sudden heart failure. Previously, we reported that increased myocardial oxidative damage, apoptosis, altered cytoskeleton and AMPK signaling proteins associated with energy deprivation in acute F− induced cardiac dysfunction. The present study was aimed to decipher the status of myocardial heat shock proteins (Hsps-Hsp27, Hsp32, Hsp40, Hsp60, Hsp70, Hsp90) and heat shock transcription factor 1 (Hsf1) in acute F−-intoxicated rats. In order to study the expression of myocardial Hsps, male Wistar rats were treated with single oral doses of 45 and 90 mg/kg F− for 24 h. The expression levels of myocardial Hsps were determined using RT-PCR, western blotting, and immunohistochemical studies. Acute F−-intoxicated rats showed elevated levels of both the transcripts and protein expression of Hsf1, Hsp27, Hsp32, Hsp60, and Hsp70 when compared to control. In addition, the expression levels of Hsp40 and Hsp90 were significantly declined in a dose-dependent fashion in F−-treated animals. Our result suggests that differential expression of Hsps in the rat myocardium could serve as a balance between pro-survival and death signal during acute F−-induced heart failure.

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Abbreviations

Hsps:

Heat shock proteins

Hsf1:

Heat shock factor 1

DAB:

3,3′-Diaminobenzidine

TMB:

3,3′,5,5′-Tetramethylbenzidine

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Acknowledgements

The authors are grateful to research fellows Ms. Kiruthika Sundarraj and Ms. Vinothini Subramaniam, Bharathiar University, Coimbatore, for their timely assistance throughout the work.

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Author notes

  1. Lakshmikanthan Panneerselvam and Azhwar Raghunath contributed equally to this work.

Authors and Affiliations

  1. Molecular Toxicology Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore, Tamil Nadu, 641 046, India

    Lakshmikanthan Panneerselvam, Azhwar Raghunath & Ekambaram Perumal

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  1. Lakshmikanthan Panneerselvam
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  2. Azhwar Raghunath
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  3. Ekambaram Perumal
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Correspondence to Ekambaram Perumal.

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The authors declare that they have no conflict of interest.

Funding information

This work was supported by the UGC-SAP DRS II:F-3-30/2013 and DST-FIST:SR/FST/LSI-618/2014, New Delhi, India, for the partial financial assistance. LP extends his thanks to the Indian Council of Medical Research, New Delhi, for financial assistance in the form of a senior research fellowship (No. 45/25/2013/BMS/TRM). AR acknowledges the UGC-BSR fellowship (UGC-BSR No. F7-25/2007) from UGC-BSR, New Delhi, India.

Electronic supplementary material

Supplementary Fig. 1

Effect of acute F− intoxication on myocardial mRNA expression of Hsf1 and Hsps in rats. (TIFF 3024 kb) (GIF 2 kb)

High resolution image (TIFF 3024 kb)

Supplementary Fig. 2

Effect of acute F− intoxication on myocardial protein expression of Hsf1 and Hsps in rats. (TIFF 3072 kb) (GIF 2 kb)

High resolution image (TIFF 3072 kb)

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Panneerselvam, L., Raghunath, A. & Perumal, E. Differential expression of myocardial heat shock proteins in rats acutely exposed to fluoride. Cell Stress and Chaperones 22, 743–750 (2017). https://doi.org/10.1007/s12192-017-0801-1

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