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The global effect of heat on gene expression in cultured bovine mammary epithelial cells

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Cell Stress and Chaperones Aims and scope

Abstract

Heat stress (HS) in hot climates is a major cause that strongly negatively affects milk yield in dairy cattle, leading to immeasurable economic loss. The heat stress response of bovine mammary epithelial cells (BMECs) is one component of the acute systemic response to HS. Gene networks of BMECs respond to environmental heat loads with both intra- and extracellular signals that coordinate cellular and whole-animal metabolism. Our experimental objective was to characterize the direct effects of heat stress on the cultured bovine mammary epithelial cells by microarray analyses. The data identified 2716 differentially expressed genes in 43,000 transcripts which were changed significantly between heat-stressed and normal bovine mammary epithelial cells (fold change ≥2, P ≤ 0.001). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that these differentially expressed genes are involved in different pathways that regulate cytoskeleton, cell cycle, and stress response processes. Our study provides an overview of gene expression profile and the interaction between gene expression and heat stress, which will lead to further understanding of the potential effects of heat stress on bovine mammary glands.

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Acknowledgments

This work received funding from the National Supporting Projects for Science and Techniques of China (2012BAD12B10) and the Natural Science Foundation of China (31372290).

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Authors and Affiliations

  1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, People’s Republic of China

    Lian Li, Yu Sun, Jie Wu, Xiaojuan Li, Man Luo & Genlin Wang

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  1. Lian Li
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  2. Yu Sun
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  3. Jie Wu
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  4. Xiaojuan Li
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  5. Man Luo
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  6. Genlin Wang
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Corresponding author

Correspondence to Genlin Wang.

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Figure S1

TGF-beta signaling pathway (List genes are noted by red starts). (GIF 232 kb)

High resolution image (TIFF 82 kb)

Table S1

Up- and down-regulated genes lists (fold change≥2) (XLS 407 kb).

Table S2

GO functional annotation chart of down-regulated genes(fold change≥3).Top ten lists of GO functional annotation chart records under biological process, cellular component and molecular function, respectively. Only those terms that have p-values≤0.05 and number of genes in each term > 5 are shown (XLS 23.0 kb).

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Li, L., Sun, Y., Wu, J. et al. The global effect of heat on gene expression in cultured bovine mammary epithelial cells. Cell Stress and Chaperones 20, 381–389 (2015). https://doi.org/10.1007/s12192-014-0559-7

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  • DOI: https://doi.org/10.1007/s12192-014-0559-7

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