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Ultrasensitive Nanosensor for Detection of Malic Acid in Tomato as Fruit Ripening Indicator

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A Correction to this article was published on 03 January 2018

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Abstract

Malic acid is an important fruit ripening indicator. Fruit industry losses every year due to non-availability of rapid technology for early detection of ripening of fruits. Therefore, nanosensor was developed for detection of malic acid concentrations in tomato at early stage of ripening before transport to the market. The enzyme NADP-malate dehydrogenase (Malic enzyme) was covalently immobilized on to screen printed carboxylated-multiwall carbon nanotubes working electrode using EDC-NHS chemistry. The enzyme electrode was characterized using scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy. The immobilized enzyme/c-MWCNT electrode was used for amperometric determination of different concentrations of malic acid in tomato using differential pulse voltammogram (DPV) at scan rate of 100 mv/s. The limit of detection of malic acid was 0.01 mM. The nanosensor showed low Km (0.12 mM), less response time (2 min), high sensitivity (0.01 mM) and better storage stability 180 days at 4 °C compared to earlier reported malate biosensor. The nanosensor was also validated at different stages of ripening of tomato using enzymatic method.

Nanosensor for detection of malic acid in tomato

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Change history

  • 03 January 2018

    The original version of this article unfortunately contained mistakes. In Figs. 2a, c and 5a in which Y-axis (Current X103) should not be written. It should only be “Current”. The correct version of Figs. 2a, c and 5a are given below.

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Acknowledgements

Anita Dalal thanks the State Government of Haryana for providing C.V. Raman fellowship to carry out this work.

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Authors and Affiliations

  1. CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi, 110007, India

    Anita Dalal & Ashok Kumar

  2. Deenbandhu Chhotu Ram University of Science and Technology, Murthal, Sonepat, Haryana, 131039, India

    Anita Dalal & J. S. Rana

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  1. Anita Dalal
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  2. J. S. Rana
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  3. Ashok Kumar
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Correspondence to Ashok Kumar.

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Anita Dalal declares that she has no conflict of interest. J. S. Rana declares that he has no conflict of interest. Ashok Kumar declares that he has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Additional information

A correction to this article is available online at https://doi.org/10.1007/s12161-017-1119-4.

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Dalal, A., Rana, J.S. & Kumar, A. Ultrasensitive Nanosensor for Detection of Malic Acid in Tomato as Fruit Ripening Indicator. Food Anal. Methods 10, 3680–3686 (2017). https://doi.org/10.1007/s12161-017-0919-x

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  • DOI: https://doi.org/10.1007/s12161-017-0919-x

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