Abstract
A novel analytical method employing immunoaffinity column (IAC) clean-up coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of ractopamine, chloramphenicol, and zeranols (α-zearalanol, β-zearalanol, zearalanone, α-zearalenol, β-zearalenol, and zearalenone) in animal-originated foods. The sample was first digested by β-glucuronidase/sulfatase and then extracted with ethyl acetate-diethyl ether (9:1, v/v). The extracted solution was evaporated to dryness and then the residue was dissolved by 2 mL of 50% acetonitrile solution. After filtration, 1 mL filtrate was diluted to 10 mL with PBS. The reconstituted solution was cleaned up with immunoaffinity column and then analyzed by high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). The established method was shown to be sensitive efficient and reliable as indicated by the linearity (r 2 ≥ 0.9994), precision (RSD ≤ 1.7%), average recovery (72.3–103.2%), and the limit of detection (0.05–0.10 μg/kg). The method can be used for determination of trace residues of ractopamine, chloramphenicol, and zeranols in animal-originated foods.
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References
Akhtar MH, Danis C, Sauve A, Barry C (1995) Gas chromatographic determination of incurred chloramphenicol residues in eggs following optimal extraction. J Chromatogr A 696(1):123–130
Allen EH (1984) Review of chromatographic methods for chloramphenicol residues in milk, eggs and tissues from food-producing animals. J Assoc Off Anal Chem 68(5):990–999
Ashwin HM, Stead SL, Taylor JC, Startin JR, Richmond SF, Homer V, Bigwood T, Sharman M (2005) Development and validation of screening and confirmatory methods for the detection of chloramphenicol and chloramphenicol glucuronide using SPR biosensor and liquid chromatography–tandem mass spectrometry. Anal Chim Acta 529(1):103–108
Beloglazova NV, Speranskaya ES, De Saeger S, Hens Z, Abe S, Goryacheva IY (2012) Quantum dot based rapid tests for zearalenone detection. Anal Bioanal Chem 403(10):3013–3024
Dickson LC, Costain R, McKenzie D, Fesser AC, Macneil JD (2009) Quantitative screening of stilbenes and zeranol and its related residues and natural precursors in veal liver by gas chromatography-mass spectrometry. J Agric Food Chem 57(15):6536–6542
Dusi G, Bozzoni E, Assini W, Tognoli N, Gasparini M, Ferretti E (2009) Confirmatory method for the determination of resorcylic acid lactones in urine sample using immunoaffinity cleanup and liquid chromatography-tandem mass spectrometry. Anal Chim Acta 637(1):47–54
He L, Su Y, Zeng Z, Liu Y, Huang X (2007) Determination of ractopamine and clenbuterol in feeds by gas chromatography-mass spectrometry. Anim Feed Sci Technol 132(3):316–323
Hutchison HE, Pinkerton PH (1962) Marrow depression due to chloramphenicol. Scott Med J 7(2):96–97
Marin DE, Taranu I, Burlacu R, Tudor DS (2010) Effects of zearalenone and its derivatives on the innate immune response of swine. Toxicon 56(6):956–963
Minervini F, Lacalandra GM, Filannino A, Nicassio M, Visconti A, Dell’Aquila ME (2010) Effects of in vitro exposure to natural levels of zearalenone and its derivatives on chromatin structure stability in equine spermatozoa. Theriogenology 73(3):392–403
Ministry of Agriculture (2002) No. 235. Bulletin of the Ministry of Agriculture of the People’s Republic of China
Nielen MWF, Van Engelen MC, Zuiderent R, Ramaker R (2007) Screening and confirmation criteria for hormone residue analysis using liquid chromatography accurate mass time-of-flight, Fourier transform ion cyclotron resonance and orbitrap mass spectrometry techniques. Anal Chim Acta 586(1):122–129
Pleadin J, Persi N, Milić D, Vahčić N (2012) Determination of residual ractopamine concentrations by enzyme immunoassay in treated pig’s tissues on days after withdrawal. Meat Sci 90(3):755–758
Qu CH, Li XL, Zhang L, Xi CX, Wang GM, Li NB, Luo HQ (2011) Simultaneous determination of cimaterol, salbutamol, terbutaline and ractopamine in feed by SPE coupled to UPLC. Chromatographia 73(3–4):243–249
Schaut A, De Saeger S, Sergent T, Schneider YJ, Larondelle Y, Pussemier L, Van Peteghem C (2008) Study of the gastrointestinal biotransformation of zearalenone in a Caco-2 cell culture system with liquid chromatographic methods. J Appl Toxicol 28(8):966–973
Spurlock ME, Cusumano JC, Ji SQ, Anderson DB, Smith CK, Hancock DL, Mills SE (1994) The effect of ractopamine on β-adrenoceptor density and affinity in porcine adipose and skeletal muscle tissue. J Anim Sci 72(1):75–82
Tsikas D (2010) Quantitative analysis of biomarkers, drugs and toxins in biological samples by immunoaffinity chromatography coupled to mass spectrometry or tandem mass spectrometry: a focused review of recent applications. J Chromatogr B 878(2):133–148
U.S. Food and Drug Administration (1999) Federal Food, Drug and Cosmetic Act (FFDCA), Section 512, 22 December 1999, FDA, Washington, DC
Urraca JL, Marazuela MD, Moreno-Bondi MC (2004) Analysis for zearalenone and α-zearalenol in cereals and swine feed using accelerated solvent extraction and liquid chromatography with fluorescence detection. Anal Chim Acta 524(1):175–183
Wong S, Silva F, Acheson J, Plant G (2013) An old friend revisited: chloramphenicol optic neuropathy. JRSM short reports 4(3):20
You LN, Li XL, Xi CX, Tang BB, Wang GM, Zhang L, Yuan ZZ, Zhao H (2012) Simultaneous determination of residues of six zeranols in eggs by high performance liquid chromatography with immunoaffinity cleanup column. Chin J Chromatogr 30(10):1021–1025
Yuan ZZ, Zhang L, Tang BB, Xi CX, Wang GM, You LN, Zhao H (2013) Determination of 16 sulfonamide residues in animal-originated foods by high performance liquid chromatography with immunoaffinity column. J Instrum Anal 32(3):478–482
Zhang MZ, Wang MZ, Chen ZL, Fang JH, Fang MM, Liu J, Yu XP (2009) Development of a colloidal gold based lateral flow immunoassay for the rapid simultaneous detection of clenbuterol and ractopamine in swine urine. Anal Bioanal Chem 395(8):2591
Zhang QJ, Peng T, Chen DD, Xie J, Wang X, Wang GM, Nie CM (2013) Determination of chloramphenicol residuces in aquatic products using immunoaffinity column cleanup and high performance liquid chromatography with ultraviolet detection. J AOAC Int 96(4):897–901
Zinedine A, Soriano JM, Molto JC, Manes J (2007) Review on the toxicity, occurrence, metabolism, detoxification, regulations and intake of zearalenone: an oestrogenic mycotoxin. Food Chem Toxicol 45(1):1–18
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Xue Sun declares that she has no conflict of interest. Qiang Tang declares that he has no conflict of interest. Xiaoli Du declares that she has no conflict of interest. Cunxian Xi declares that he has no conflict of interest. Bobin Tang declares that he has no conflict of interest. Guomin Wang declares that he has no conflict of interest. Hua Zhao declares that he has no conflict of interest.
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Sun, X., Tang, Q., Du, X. et al. Simultaneous Determination of Ractopamine, Chloramphenicol, and Zeranols in Animal-Originated Foods by LC-MS/MS Analysis with Immunoaffinity Clean-up Column. Food Anal. Methods 10, 3239–3246 (2017). https://doi.org/10.1007/s12161-017-0858-6
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DOI: https://doi.org/10.1007/s12161-017-0858-6