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Simultaneous Determination of Cimaterol, Salbutamol, Terbutaline and Ractopamine in Feed by SPE Coupled to UPLC

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Abstract

An ultra performance liquid chromatography (UPLC) method was established for the simultaneous determination of cimaterol, salbutamol, terbutaline, and ractopamine in feed. Target compounds were extracted with methanol and centrifuged. The supernatant was then transferred and concentrated, and applied to a solid phase extraction MCX cartridge for clean-up before UPLC analysis. The calibration curves for the four β-agonists were good linear in concentration range of 0.05–1.0 μg mL−1 with the correlation coefficients (r) over 0.999. The average recoveries were in the range of 90.1–101.4% with relative standard deviation values lower than 8.0%.

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Acknowledgments

This work was supported by the General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China Research Project (NO. 2008IK167) and the National Natural Science Foundation of China (20975083). The research was finished in the Technology Center Laboratory of Chongqing Entry-Exit Inspection and Quarantine Bureau. We thank the Lab for providing us with pure standards of β-agonists, feed samples, and the analytical instrument in this study.

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Authors and Affiliations

  1. Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China

    Chun Hua Qu, Nian Bing Li & Hong Qun Luo

  2. Chongqing Entry-Exit Inspection and Quarantine Bureau, Chongqing Import and Export Food Safety Engineering Technology Research Center, Chongqing, 400020, China

    Xian Liang Li, Lei Zhang, Cun Xian Xi & Guo Ming Wang

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  1. Chun Hua Qu
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  2. Xian Liang Li
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  3. Lei Zhang
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  7. Hong Qun Luo
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Correspondence to Hong Qun Luo.

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Qu, C.H., Li, X.L., Zhang, L. et al. Simultaneous Determination of Cimaterol, Salbutamol, Terbutaline and Ractopamine in Feed by SPE Coupled to UPLC. Chromatographia 73, 243–249 (2011). https://doi.org/10.1007/s10337-010-1873-6

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  • DOI: https://doi.org/10.1007/s10337-010-1873-6

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