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Electrochemical Immunosensor Based on the Chitosan-Magnetic Nanoparticles for Detection of Tetracycline

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Abstract

In this paper, a low-cost, simple, and highly sensitive electrochemical immunosensor was fabricated for the tetracycline detection based on gold electrode-modified carboxyl-Fe3O4 nanoparticle (MNPs) by chitosan (CS) as linker. The anti-tetracycline monoclonal antibody (Ab) was immobilized on the modified electrode surface. The binding of tetracycline to Ab was analyzed by differential pulse voltammetry. Here, MNPs were used as the signal amplifier to improve the sensitivity of the immunosensor. The stepwise assembly process of the electrochemical immunosensor was characterized by cyclic voltammetry and electrochemical impedance spectroscopy. Under the optimum operating conditions, the fabricated immunosensor showed a linear current response to the target concentration in the range from 0.08 to 1 ng/mL with a lower detection limit of 0.0321 ng/mL (S/N = 3). It was successfully applied to the detection of tetracycline in milk. Enzyme-linked immunoassay analysis was also conducted to detect tetracycline in the same samples for demonstrating the applicability of the electrochemical immunosensor.

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Funding

This work was financially supported by the Hunan Provincial Natural Science Foundation of China (No. 2015JJ3077), Special Fund for Agro-Scientific Research in the Public Interest (No. 201303084), and “1515” talent cultivation plan of Hunan Agricultural University.

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Authors and Affiliations

  1. College of Food Science and Technology, Hunan Agricultural University, Furong District, Changsha, 410128, People’s Republic of China

    Xia Liu, Shu Zheng, Yuxin Hu, Zongjun Li, Fang Luo & Zao He

  2. Hunan Province Key Laboratory of Food Science and Biotechnology, Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Changsha, 410128, People’s Republic of China

    Xia Liu, Shu Zheng, Yuxin Hu, Zongjun Li, Fang Luo & Zao He

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  1. Xia Liu
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  2. Shu Zheng
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  3. Yuxin Hu
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  4. Zongjun Li
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  5. Fang Luo
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Correspondence to Xia Liu.

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Xia Liu declares that she has no conflict of interest. Shu Zheng declares that she has no conflict of interest. Yuxin Hu declares that she has no conflict of interest. Zongjun Li declares that he has no conflict of interest. Fang Luo declares that she has no conflict of interest. Zao He declares that she has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Liu, X., Zheng, S., Hu, Y. et al. Electrochemical Immunosensor Based on the Chitosan-Magnetic Nanoparticles for Detection of Tetracycline. Food Anal. Methods 9, 2972–2978 (2016). https://doi.org/10.1007/s12161-016-0480-z

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  • DOI: https://doi.org/10.1007/s12161-016-0480-z

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