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Electrochemical aptasensor for tetracycline detection

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Abstract

An electrochemical aptasensor was developed for the detection of tetracycline using ssDNA aptamer that selectively binds to tetracycline as recognition element. The aptamer was highly selective for tetracycline which distinguishes minor structural changes on other tetracycline derivatives. The biotinylated ssDNA aptamer was immobilized on a streptavidin-modified screen-printed gold electrode, and the binding of tetracycline to aptamer was analyzed by cyclic voltammetry and square wave voltammetry. Our results showed that the minimum detection limit of this sensor was 10 nM to micromolar range. The aptasensor showed high selectivity for tetracycline over the other structurally related tetracycline derivatives (oxytetracycline and doxycycline) in a mixture. The aptasensor developed in this study can potentially be used for detection of tetracycline in pharmaceutical preparations, contaminated food products, and drinking water.

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Acknowledgments

This work was supported by the Industrial Technology Development, Ministry of knowledge Economy (10032113). The authors express their gratitude for this support.

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Authors and Affiliations

  1. School of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-gu, Seoul, 136-701, Republic of Korea

    Yoon-Jin Kim, Yeon Seok Kim, Javed H. Niazi & Man Bock Gu

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  1. Yoon-Jin Kim
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  2. Yeon Seok Kim
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  3. Javed H. Niazi
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  4. Man Bock Gu
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Correspondence to Man Bock Gu.

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Kim, YJ., Kim, Y.S., Niazi, J.H. et al. Electrochemical aptasensor for tetracycline detection. Bioprocess Biosyst Eng 33, 31–37 (2010). https://doi.org/10.1007/s00449-009-0371-4

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  • DOI: https://doi.org/10.1007/s00449-009-0371-4

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