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Lignin Removal and Cellulose Digestibility Improved by Adding Antioxidants and Surfactants to Organosolv Pretreatment of Sugarcane Bagasse

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Abstract

Annually, tons of sugarcane bagasse are generated in the sugar and alcohol industries. This biomass has great potential in the use of converting cellulose into glucose, an energy currency for various biotechnological processes. However, lignin content is a limiting factor in cellulose accessibility. This study aimed to improve lignin removal by evaluating the additive's effect on biomass pretreatment. The additives were tert-butylhydroquinone, 3-tert-butyl-4-hydroxyanisole, methyl 3,4,5-trihydroxybenzoate; surfactants Tween 20, Tween 80, and dimethyl sulfoxide (DMSO). The antioxidants collaborated with lignin removal; the 3-tert-butyl-4-hydroxyanisole reached 71% of lignin removal. Inherent to the pretreatment, tert-butylhydroquinone showed 23.53% and 89.54% of cellulose and hemicellulose removal, respectively. Dioxane extraction compounds from the pretreated biomass showed an increased amount using additives, suggesting more compounds adsorbed on the material surface. All the antioxidants/surfactants applied to the organosolv pretreatment improved enzymatic hydrolysis, reaching 98.9% cellulose into glucose conversion (Tween 80). The use of chemical compounds during pretreatment beneficed the removal of lignin from biomass and consequently the cellulose hydrolysis.

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Abbreviations

TB:

Tert-butylhydroquinone

TH:

3-Tert-butyl-4-hydroxyanisole

MT:

Methyl 3,4,5-trihydroxybenzoate

T2:

Tween 20

T8:

Tween 80

DS:

Dimethyl sulfoxide – DMSO

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Acknowledgements

The authors would like to thank the Brazilian Council for Research and Development – CNPq (process number: 401900/2016-9) and São Paulo Research Foundation (process number 2017/22401-8).

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Authors and Affiliations

  1. Institute For Research in Bioenergy (IPBEN), São Paulo State University (UNESP), R. 10, 2527, Santana, Rio Claro, SP, 13500-230, Brazil

    Alison Andrei Schmatz & Michel Brienzo

  2. School of Pharmaceutical Sciences (FCF), Department of Bioprocess Engineering and Biotechnology, São Paulo State University (UNESP), Araraquara, SP, Brazil

    Fernando Masarin

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  1. Alison Andrei Schmatz
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Correspondence to Michel Brienzo.

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Schmatz, A.A., Masarin, F. & Brienzo, M. Lignin Removal and Cellulose Digestibility Improved by Adding Antioxidants and Surfactants to Organosolv Pretreatment of Sugarcane Bagasse. Bioenerg. Res. 15, 1107–1115 (2022). https://doi.org/10.1007/s12155-021-10367-0

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