Abstract
Dendrocalamus sinicus, which is the largest bamboo species in the world, has broad prospects in the fields of bioenergy and biorefinery application. In this study, dewaxed D. sinicus samples were sequentially treated with 80 % ethanol containing 0.025 M HCl, 80 % ethanol containing 0.5 % NaOH, and aqueous alkaline solutions (containing 2.0, 5.0, and 8.0 % NaOH, respectively) at 75 °C for 4 h, in which 9.63, 8.71, 21.83, 21.09, and 13.09 % of the original lignin were isolated, respectively. The lignin fractions obtained were comparatively characterized by chemical composition, molecular weights, and structural features by wet chemical and instrumental analysis methods. It was found that the bamboo lignin fractions isolated by ethanol had lower weight-average molecular weights (1,360–1,380 g mol−1) and contained much higher amounts of associated hemicelluloses, while the lignin fractions isolated by aqueous alkaline solutions had higher weight-average molecular weights (5,300–6,040 g mol−1) and contained lower amounts of associated hemicelluloses. Spectroscopy analyses indicated that the bamboo lignin was a typical grass lignin, consisting of p-hydroxyphenyl (H), guaiacyl (G), and syringyl (S) units. A small percentage of the lignin side-chain was found to be acetylated at the γ-carbon, predominantly at syringyl units. The major interunit linkages present in the bamboo lignin obtained were β-O-4′ aryl ether linkages, together with lower amounts of β-β′, β-5′, and β-1′ linkages.
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Acknowledgments
The authors are grateful for the financial support from Major State Basic Research Projects of China (973-2010CB732204), the National Natural Science Foundation of China (31260165, 31110103902), Education Department of Yunnan Province, China (2011Z040), and State Key Laboratory of Pulp and Paper Engineering, South China University of Technology (201132).
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Shi, ZJ., Xiao, LP., Deng, J. et al. Isolation and Structural Characterization of Lignin Polymer from Dendrocalamus sinicus . Bioenerg. Res. 6, 1212–1222 (2013). https://doi.org/10.1007/s12155-013-9321-8
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DOI: https://doi.org/10.1007/s12155-013-9321-8