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NMR assignments of SPOC domain of the human transcriptional corepressor SHARP in complex with a C-terminal SMRT peptide

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Abstract

The transcriptional corepressor SMRT/HDAC1-associated repressor protein (SHARP) recruits histone deacetylases. Human SHARP protein is thought to function in processes involving steroid hormone responses and the Notch signaling pathway. SHARP consists of RNA recognition motifs (RRMs) in the N-terminal region and the spen paralog and ortholog C-terminal (SPOC) domain in the C-terminal region. It is known that the SPOC domain binds the LSD motif in the C-terminal tail of corepressors silencing mediator for retinoid and thyroid receptor (SMRT)/nuclear receptor corepressor (NcoR). We are interested in delineating the mechanism by which the SPOC domain recognizes the LSD motif of the C-terminal tail of SMRT/NcoR. To this end, we are investigating the tertiary structure of the SPOC/SMRT peptide using NMR. Herein, we report on the 1H, 13C and 15N resonance assignments of the SPOC domain in complex with a SMRT peptide, which contributes towards a structural understanding of the SPOC/SMRT peptide and its molecular recognition.

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Acknowledgments

This work was supported in part by a Grant-in-Aid for Scientific Research on Innovative Areas, “Structural Cell Biology” and “Transient Macromolecular Complex” from the Japanese Ministry of Education, Culture, Sports, and Technology (to M.M.), and the Asian Human Resources Fund of Tokyo Metropolitan Government (under Project Asian Network for Major Cities 21) (to Y.I. and M.M.). T.K. is a recipient of a Sasagawa Scientific Research Grant. We thank RIKEN SSBC for help with the 900 MHz NMR measurements.

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  1. Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minamiosawa, Hachioji, 192-0397, Japan

    Suzuka Mikami, Teppei Kanaba, Yutaka Ito & Masaki Mishima

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  1. Suzuka Mikami
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  2. Teppei Kanaba
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  3. Yutaka Ito
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  4. Masaki Mishima
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Correspondence to Masaki Mishima.

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Mikami, S., Kanaba, T., Ito, Y. et al. NMR assignments of SPOC domain of the human transcriptional corepressor SHARP in complex with a C-terminal SMRT peptide. Biomol NMR Assign 7, 267–270 (2013). https://doi.org/10.1007/s12104-012-9424-8

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  • DOI: https://doi.org/10.1007/s12104-012-9424-8

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