Abstract
We present here the backbone and side-chain NMR assignments of YFP Venus, a 238-residue protein that emits yellow fluorescence in its native state. Venus is a variant of the green fluorescent protein (GFP), which has improved chromophore maturation and brightness, and the photochemistry and photophysics of which are insensitive to experimental conditions, such as the pH value and buffer content, making it a favourable biomarker.
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Acknowledgments
STDH is a recipient of a Netherlands Ramsay and a Human Frontier Science Program Long-term Fellowship (LT0798/2005). CB is holding a Studienstiftung des Deutschen Volkes scholarship and is supported by the Erasmus/Sokrates program. LDC is a NH&MRC C.J. Martin Fellow. CMD acknowledges funding from the Wellcome and Leverhulme Trusts. We thank Prof. Atsushi Miyawaki for providing the DNA plasmid of Venus, Dr. John Christodoulou for helpful discussion, and the staff of the Biomolecular NMR Facility, Department of Chemistry, University of Cambridge. Financial support by the Access to Research Infrastructures activity in the 6th Framework Program of the EC (Contract # RII3-026145, EU-NMR) for conducting the research at CERM is gratefully acknowledged.
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Hsu, ST.D., Behrens, C., Cabrita, L.D. et al. 1H, 15N and 13C assignments of yellow fluorescent protein (YFP) Venus. Biomol NMR Assign 3, 67–72 (2009). https://doi.org/10.1007/s12104-009-9143-y
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DOI: https://doi.org/10.1007/s12104-009-9143-y