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Phytase, Phosphatase Activity and P-Nutrition of Soybean as Influenced by Inoculation of Bacillus

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Abstract

The efficiency of different Bacillus isolates on rhizosphere soil enzyme activities and P-nutrition of soybean was carried out under microcosm conditions. Significant increase in enzyme activities viz., fluorescein diacetate activity, phosphatase and phytase activity and consequent effects on P-nutrition were observed with the inoculation of Bacillus isolates over uninoculated control. Among the isolates, BD-3-1B, KHBD-6, BDKH-3, Bacillus amyloliquefacians, and Bacillus cereus were found to be promising. The phytic acid-P as a percentage of total P content in soybean seeds decreased with the inoculation of Bacillus isolates as compared to un-inoculated control. A decrease in phytic-P in soybean seeds not only results in better digestibility and increased feed efficiency. Pearson correlation studies revealed a significant positive association between acid, alkaline phosphatases, phytase activity on available P content in soil and P content in seeds with the inoculation of Bacillus isolates, indicating role of these enzymes in P mobilization and acquisition by soybean.

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Authors and Affiliations

  1. Directorate of Soybean Research (ICAR), Khandwa Road, Indore, 452 001, Madhya Pradesh, India

    A. Ramesh, Sushil K. Sharma, O. P. Joshi & I. R. Khan

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  1. A. Ramesh
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  2. Sushil K. Sharma
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  3. O. P. Joshi
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  4. I. R. Khan
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Correspondence to Sushil K. Sharma.

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Ramesh, A., Sharma, S.K., Joshi, O.P. et al. Phytase, Phosphatase Activity and P-Nutrition of Soybean as Influenced by Inoculation of Bacillus . Indian J Microbiol 51, 94–99 (2011). https://doi.org/10.1007/s12088-011-0104-7

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  • DOI: https://doi.org/10.1007/s12088-011-0104-7

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