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Phosphorus Mobilization from Native Soil P-Pool upon Inoculation with Phytate-Mineralizing and Phosphate-Solubilizing Bacillus aryabhattai Isolates for Improved P-Acquisition and Growth of Soybean and Wheat Crops in Microcosm Conditions

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Abstract

Microbial transformation of both inorganic and organic forms of soil phosphorus to plant available P is an important trait contributing to plant P-nutrition and growth promotion. The present study was undertaken to evaluate phytate-mineralizing and phosphate-solubilizing Bacillus aryabhattai isolates on plant growth promotion, rhizosphere properties, P-mobilization from native P-pool of soil and acquisition by soybean and wheat crops in Vertisols of central India. Under microcosm conditions, a significant increase in plant growth parameters and plant P content of soybean (R 5 stage) and wheat (panicle initiation stage) crops as a result of inoculation was recorded over un-inoculated control. Similarly, rhizosphere soil properties like activities of fluorescein diacetate, acid and alkaline phosphatase, phytase and microbial biomass-P were also increased with inoculation. These properties were found to be higher with inoculation of isolate MDSR14 followed by MDSR7. At maturity, inoculation with isolates MDSR7 and MDSR14 significantly increased shoot and seed weight, P content and decreased Phytic-P expressed as a percentage of total P content. There was a concomitant depletion in native organic P and acid extractable-P and increase in inorganic P in rhizosphere soil over un-inoculated control indicating mobilization of native unavailable organic P and inorganic insoluble P-pool of soil to available P. Moreover, inoculation also decreased the level of Phytic-P expressed as a percentage of total P content in seeds in soybean and wheat crops as compared to un-inoculated control. The decrease in Phytic-P results in better digestibility and increased feed efficiency and has implication in availability of minerals to human being and animals. The results suggest that B. aryabhattai isolates (MDSR14 and MDSR7) have potential to mobilize native soil P-pool and improve growth, yield and P-assimilation by soybean and wheat crops.

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Acknowledgments

The authors are grateful to Dr. S. Shivaji, Scientist G (Director—grade Scientist), Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India for carrying out 16S rRNA gene sequencing of the isolate MDSR14. We also thank Mr. I.R. Khan and Mr. Gorelal for their technical assistance during experimentation.

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The authors declared that they have no conflict of interest.

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Author notes

  1. Sushil K. Sharma

    Present address: National Bureau of Agriculturally Important Microorganisms (ICAR), Kushmaur, Post Bag No. 6, Mau Nath Bhanjan, 275 103, Uttar Pradesh, India

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  1. Directorate of Soybean Research (DSR-ICAR), Khandwa Road, Indore, 452 001, Madhya Pradesh, India

    Aketi Ramesh, Sushil K. Sharma, Namrata Yadav & O. P. Joshi

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Ramesh, A., Sharma, S.K., Yadav, N. et al. Phosphorus Mobilization from Native Soil P-Pool upon Inoculation with Phytate-Mineralizing and Phosphate-Solubilizing Bacillus aryabhattai Isolates for Improved P-Acquisition and Growth of Soybean and Wheat Crops in Microcosm Conditions. Agric Res 3, 118–127 (2014). https://doi.org/10.1007/s40003-014-0105-y

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