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Comparative analysis of gene expression profiles of OPN signalling pathway in four kinds of liver diseases

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Abstract

To explore the relevance of OPN signalling pathway to the occurrence and development of nonalcoholic fatty liver disease (NAFLD), liver cirrhosis (LC), hepatic cancer (HC) and acute hepatic failure (AHF) at transcriptional level, Rat Genome 230 2.0 Array was used to detect expression profiles of OPN signalling pathway-related genes in four kinds of liver diseases. The results showed that 23, 33, 59 and 74 genes were significantly changed in the above four kinds of liver diseases, respectively. H-clustering analysis showed that the expression profiles of OPN signalling-related genes were notably different in four kinds of liver diseases. Subsequently, a total of above-mentioned 147 genes were categorized into four clusters by k-means according to the similarity of gene expression, and expression analysis systematic explorer (EASE) functional enrichment analysis revealed that OPN signalling pathway-related genes were involved in cell adhesion and migration, cell proliferation, apoptosis, stress and inflammatory reaction, etc. Finally, ingenuity pathway analysis (IPA) software was used to predict the functions of OPN signalling-related genes, and the results indicated that the activities of ROS production, cell adhesion and migration, cell proliferation were remarkably increased, while that of apoptosis, stress and inflammatory reaction were reduced in four kinds of liver diseases. In summary, the above physiological activities changed more obviously in LC, HC and AHF than in NAFLD.

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Acknowledgements

This study was funded by the National Natural Science Foundation Project of China (no. 81200317), the Natural Science Foundation of China (no. 31572270), the Major Scientific and Technological Projects of Henan (no. 111100910600), the doctoral Scientific Research Start-up Foundation of Henan Normal University (no. 11128), and the Youth Science Foundation of Henan Normal University (no. 2013qk12). We thank Mr Yunpeng Hao and Mr Jia Zhi for their hard work in preparing animal models, Dr Cuifang Chang for her data analysis, and Ms Jun Li for her hard work during revising the manuscript.

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Authors and Affiliations

  1. College of Life Science, Henan Normal University, Xinxiang, 453007, Henan, China

    GAIPING WANG, SHASHA CHEN, CONGCONG ZHAO, XIAOFANG LI, WEIMING ZHAO & JING YANG

  2. State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University, Xinxiang, China

    GAIPING WANG, SHASHA CHEN, CONGCONG ZHAO, XIAOFANG LI, WEIMING ZHAO, JING YANG, CUIFANG CHANG & CUNSHUAN XU

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  1. GAIPING WANG
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  2. SHASHA CHEN
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  3. CONGCONG ZHAO
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  8. CUNSHUAN XU
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Correspondence to CUNSHUAN XU.

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[Wang G., Chen S., Zhao C., Li X., Zhao W., Yang J., Chang C. and Xu C. 2016 Comparative analysis of gene expression profiles of OPN signalling pathway in four kinds of liver diseases. J. Genet. 95, xx–xx]

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WANG, G., CHEN, S., ZHAO, C. et al. Comparative analysis of gene expression profiles of OPN signalling pathway in four kinds of liver diseases. J Genet 95, 741–750 (2016). https://doi.org/10.1007/s12041-016-0673-7

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