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Bioremediation potential of biofilm forming multi-metal resistant marine bacterium Pseudomonas chengduensis PPSS-4 isolated from contaminated site of Paradip Port, Odisha

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Abstract

Biofilm forming and heavy metal resistant marine bacterial strain Pseudomonas chengduensis PPSS-4 was isolated from the contaminated marine sediment of Paradip Port, Odisha, India. The strain showed biofilm formation up to 100 mg/L of multi-metal [Pb(II), Cr(VI), and Cd(II)] supplementation in the culture medium. Scanning electron microscopy (SEM) showed aggregation of rod-shaped cells in the extracellular polymeric substance (EPS) matrix of biofilm. Confocal laser scanning microscopy (CLSM) exhibited a higher nucleic acid to the α-polysaccharide ratio in the biofilm, and the observed thickness was ~21 µm. The metal uptake potential of biofilm culture was higher than planktonic culture both in single and multi-metal solutions. FESEM-EDS analysis revealed the sequestration of multi-metals by bacterial cells and biofilm-EPS. FTIR analysis of bacterial EPS further ensured the interaction of functional groups such as –OH, –NH, and P=O with the metal ions. The maximum removal of Pb, Cr, and Cd by the bacterial biomass was observed at 37°C within 4 h of contact time at pH 6, and 4% salinity for Pb and Cr, and 6% salinity for Cd. The present study revealed that the marine bacterium P. chengduensis PPSS-4 can remove multi-metals, and this bacterium could be efficiently utilized for the remediation of heavy metals in the contaminated environment.

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Acknowledgements

The authors acknowledge NIT Rourkela, Odisha, for providing the research facility. MP acknowledge the Department of Science & Technology, Govt. of India, for the INSPIRE Award (No. DST/INSPIRE Fellowship/2017/IF170195) for the doctoral study.

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  1. Laboratory of Environmental Microbiology and Ecology (LEnME), Department of Life Science, National Institute of Technology, Rourkela, Odisha, 769 008, India

    Monika Priyadarshanee & Surajit Das

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  1. Monika Priyadarshanee
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MP executed the experiments and drafted the manuscript. SD conducted the field visit for sampling, designed the research, secured internal funding, supervised the experiments and finalized the manuscript.

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Correspondence to Surajit Das.

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Communicated by Maripi Dileep

This article is part of the topical collection: Advances in Coastal Research.

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Priyadarshanee, M., Das, S. Bioremediation potential of biofilm forming multi-metal resistant marine bacterium Pseudomonas chengduensis PPSS-4 isolated from contaminated site of Paradip Port, Odisha. J Earth Syst Sci 130, 125 (2021). https://doi.org/10.1007/s12040-021-01627-w

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