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Metal biosorption by magnetotactic bacteria isolated from fresh water sediments and characterization of extracted magnetosomes

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Abstract

The focus of the present study is to isolate and identify magnetotactic bacteria from fresh water sediments in Salem region, Tamil Nadu. Fresh water sediments were collected and characterized by X-ray diffraction to detect the crystalline nature of particles. Totally 12 isolates were screened for the metal biosorption efficiency in modified nutrient agar plates with 10 mg (lower concentration) of four different metals viz., manganese chloride, zinc sulphate, copper sulphate and potassium dichromate. Followed by testing their ability to tolerate higher concentration of metals viz., 20 mg, 30 mg, 50 mg, 70 mg, 90 mg and 150 mg/50 ml was analyzed. Only four bacteria survived the highest concentration of manganese and zinc (3000 µg/ml), CuSO4 and K2Cr2O7 at a concentration of 1400 µg/ml and 1800 µg/ml, respectively. The four bacterial strains Stenotrophomonas maltophilia, two Pseudomonas aeruginosa strains and Achromobacter xylosoxidans were grown in modified nutrient broth (NB) and Luria Bertani (LB) incorporated with metals such as manganese, zinc, copper and chromium and tested for their efficacy to sustain metal stress. Since the two bacterial strains (SBY and KY1) were able to grow in both medium with a potential to with stand higher metal concentration these strains were further studied. A metal tolerant magnetotactic bacterial strain Pseudomonas aeruginosa SBY was confirmed by TEM analysis to detect the accumulated metal within the cell. As bacterial strains were capable of tolerating higher concentration of metal, they may have a vital role in environmental bioremediation.

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Acknowledgements

The authors would like to thank Periyar University for providing laboratory facilities and university research fellowship to perform this research work. The authors also sincerely show gratitude to DST-FIST, New Delhi, India for granting sophisticated instrumentation with reference No. SR/FST/LSI-640/2015 (C) dated 30/5/2016.

Funding

The authors thank Periyar University, Salem for Providing University research fellowship to carry out this research work and the authors also sincerely show gratitude to DST-FIST, New Delhi, India for granting sophisticated instrumentation facility with reference No. SR/FST/LSI-640/2015 (C) dated May 30, 2016.

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Authors and Affiliations

  1. Department of Microbiology, School of Biosciences, Periyar University, Salem, Tamil Nadu, 636011, India

    Jamunadevi Jayaraman, Santhosh Sigamani & Dhandapani Ramamurthy

Authors
  1. Jamunadevi Jayaraman
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  2. Santhosh Sigamani
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  3. Dhandapani Ramamurthy
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Contributions

Dr. R. Dhandapani and Dr. J. Jamunadevi conceived and designed the experiments. Dr. J. Jamunadevi performed the experimental analysis. Dr. R. Dhandapani analyzed the data, provided resources for the research work and Dr. S. Santhosh contributed with statistical analysis and interpretation of data.

Corresponding author

Correspondence to Dhandapani Ramamurthy.

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This study is on isolation of fresh water bacteria and its applications, no ethical approval was obtained as there were no human/animal participants involved in this study.

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I Dr. Dhandapani Ramamurthy along with all coauthors agree to participate in this research study.

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Communicated by Erko Stackebrandt.

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Jayaraman, J., Sigamani, S. & Ramamurthy, D. Metal biosorption by magnetotactic bacteria isolated from fresh water sediments and characterization of extracted magnetosomes. Arch Microbiol 203, 5951–5962 (2021). https://doi.org/10.1007/s00203-021-02534-w

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  • DOI: https://doi.org/10.1007/s00203-021-02534-w

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