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Evaluation of Motor Neuron-Like Cell Differentiation of hEnSCs on Biodegradable PLGA Nanofiber Scaffolds

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Abstract

Human endometrium is a high-dynamic tissue that contains human endometrial stem cells (hEnSCs) which can be differentiated into a number of cell lineages. The differentiation of hEnSCs into many cell lineages such as osteoblast, adipocyte, and neural cells has been investigated previously. However, the differentiation of these stem cells into motor neuron-like cells has not been investigated yet. Different biochemical and topographical cues can affect the differentiation of stem cells into a specific cell. The aim of this study was to investigate the capability of hEnSCs to be differentiated into motor neuron-like cells under biochemical and topographical cues. The biocompatible and biodegradable poly(lactic-co-glycolic acid) (PLGA) electrospun nanofibrous scaffold was used as a topographical cue. Human EnSCs were cultured on the PLGA scaffold and tissue culture polystyrene (TCP), then differentiation of hEnSCs into motor neuron-like cells under induction media including retinoic acid (RA) and sonic hedgehog (Shh) were evaluated for 15 days. The proliferation rate of cells was assayed by using MTT assay. The morphology of cells was studied by scanning electron microscopy imaging, and the expression of motor neuron-specific markers by real-time PCR and immunocytochemistry. Results showed that survival and differentiation of hEnSCs into motor neuron-like cells on the PLGA scaffold were better than those on the TCP group. Taken together, the results suggest that differentiated hEnSCs on PLGA can provide a suitable, three-dimensional situation for neuronal survival and outgrowth for regeneration of the central nervous system, and these cells may be a potential candidate in cellular therapy for motor neuron diseases.

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Acknowledgments

We thank the Iran National Science Foundation (INSF) for the financial support (grant number 92004774) and Tehran University of Medical Sciences for this research.

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Authors and Affiliations

  1. Brain and Spinal Cord Injury Research Center (BASIR), Tehran University of Medical Sciences, Keshavarz Boulevard, Gharib Street, 6114185, Tehran, Iran

    Somayeh Ebrahimi-Barough, Abbas Norouzi Javidan, Hoshangh Saberi, Sadegh Shirian & Jafar Ai

  2. Department of Tissue Engineering, Faculty of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Somayeh Ebrahimi-Barough & Jafar Ai

  3. Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran

    Mohammad Tghi Joghataei & Faezeh Faghihi

  4. Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

    Mohammad Tghi Joghataei

  5. Umbilical Cord Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Reza Rahbarghazi

  6. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Esmaeil Mirzaei

  7. Dentistry Faculty, Tehran University of Medical Sciences, Tehran, Iran

    Armin Ai

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  1. Somayeh Ebrahimi-Barough
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  2. Abbas Norouzi Javidan
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Correspondence to Jafar Ai.

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Ebrahimi-Barough, S., Norouzi Javidan, A., Saberi, H. et al. Evaluation of Motor Neuron-Like Cell Differentiation of hEnSCs on Biodegradable PLGA Nanofiber Scaffolds. Mol Neurobiol 52, 1704–1713 (2015). https://doi.org/10.1007/s12035-014-8931-2

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  • DOI: https://doi.org/10.1007/s12035-014-8931-2

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