Abstract
Oligodendrocytes are myelinating cells in the central nervous system that form the myelin sheath of axons to support rapid nerve conduction. Human endometrial stromal cells (EnSCs) are the abundant and easy available source for cell replacement therapy. In the present study, the EnSCs were coaxed to oligodendrocyte progenitor programming by induction of neuronal condition media, including bFGF, epidermal growth factor, and platelet-derived growth factor (PDGF)-AA signaling molecules as well as triiodothyronine. Differentiated cells were analyzed for expression of oligodendrocytic markers by quantitative reverse transcription PCR and immunocytochemistry. The results showed the expression of oligodendrocyte lineage markers such as nestin, PDGF receptor alpha (PDGFRα), Sox10, and Olig2 in the level of mRNAs. The expression of nestin and PDGFRα increased after 8 days posttreatment. Interestingly, the expression of nestin and PDGFRα genes at the levels of mRNA and proteins decreased 24 days after induction. The expression of A2B5, O4, and Olig2 proteins in EnSCs was confirmed using immunocytochemistry. The results confirmed that EnSCs could response to the signaling molecules which routinely applied for oligodendrocyte differentiation. Here for the first time, we demonstrated that EnSCs could be programmed into oligodendrocyte progenitor cells and may convince to consider these cells as suitable source for cell therapy of neurodegenerative diseases.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ai J, Shahverdi AR, Ebrahimi S et al (2012) Derivation of adipocytes from human endometrial stem cells (EnSCs). J Reprod Infertil 13:151–157
Ben-Hur T, Einstein O, Mizrachi-Kol R et al (2003) Transplanted multipotential neural precursor cells migrate into the inflamed white matter in response to experimental autoimmune encephalomyelitis. Glia 41:73–80
Chua SJ, Bielecki R, Wong CJ et al (2009) Neural progenitors, neurons and oligodendrocytes from human umbilical cord blood cells in a serum- free, feeder free cell culture. Biochem Biophys Res Commun 379:217–221
Czepiel M, Balasubramaniyan V, Schaafsma W et al (2011) Differentiation of induced pluripotent stem cells into functional oligodendrocytes. Glia 59:882–892
Dimitrov R, Timeva T, Kyurkchiev D et al (2008) Characterization of clonogenic stromal cells isolated from human endometrium. Reproduction 135:551–558
Durand B, Raff M (2000) A cell-intrinsic timer that operates during oligodendrocyte development. Bioessays 22:64–71
Einstein O, Fainstein N, Vaknin I et al (2007) Neural precursors attenuate autoimmune encephalomyelitis by peripheral immunosuppression. Ann Neurol 61:209–218
Franklin RJ, Ffrench-Constant C (2008) Remyelination in the CNS: from biology to therapy. Nat Rev Neurosci 9:839–855
Gargett CE (2006) Identification and characterization of human endometrial stem/progenitor cells. Aust N Z Obstet Gynaecol 46:250–253
Gargett CE (2007) Uterine stem cells: what is the evidence? Hum Reprod Updat 13:87–101
Gargett C, Schwab K, Zillwood R et al (2009) Isolation and culture of epithelial progenitors and mesenchymal stem cells from human endometrium. Biol Reprod 80:1136–1145
Gargett C, Masuda H (2010) Adult stem cells in the endometrium. Mol Hum Reprod 16:818–834
Hendrickson ML, Rao AJ, Demerdash O et al (2011) Expression of nestin by neural cells in the adult rat and human brain. PLoS One 6:1–15
Jabbour HN, Kelly RW, Fraser HM et al (2006) Endocrine regulation of menstruation. Endocr Rev 27:17–46
Kagawa T, Wada T, Ikenaka K (2001) Regulation of oligodendrocyte development. Microse Res Tech 52:740–745
Kennea N, Waddington S, Chan J et al (2009) Differentiation of human fetal mesenchymal stem cells into cells with an oligodendrocyte phenotype. Cell Cycle 8:1069–1079
Kuhlbrodt K, Herbarth B, Sock E et al (1998) Sox10 a novel transcriptional modulator in glial cells. J Neurosci 18:237–250
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method. Methods 25:402–408
Martino G, Franklin RJ, Van AB et al (2010) Stem cell transplantation in multiple sclerosis: current status and future prospects. Nat Rev Neurol 6:247–255
Meng X, Ichim TE, Zhong J et al (2007) Endometrial regenerative cells: a novel stem cell population. J Transl Med 5:57–67
Mobarakeh TZ, Ai J, Yazdani F et al (2012) Human endometrial stem cells as a new source for programming to neural cells. Cell Biol Int Rep 19:7–14
Nistor GI, Totoiu MO, Haque N et al (2005) Human embryonic stem cells differentiate into oligodendrocytes in high purity and myelinate after spinal cord transplantation. Glia 49:385–396
Ogawa S, Tokumoto Y, Miyake J et al (2011) Induction of oligodendrocyte differentiation from adult human fibroblast-derived induced pluripotent stem cells. In Vitro Cell Dev Biol Animal 47:464–469
Patel AN, Park E, Kuzman M et al (2008) Multipotent menstrual blood stromal stem cells: isolation, characterization, and differentiation. Cell Transplant 17:303–311
Pluchino S, Quattrini A, Brambilla E et al (2003) Injection of adult neurospheres induces recovery in a chronic model of multiple sclerosis. Nature 422:688–694
Reubinoff BE, Itsykson P, Turetsky T et al (2001) Neural progenitors from human embryonic stem cells. Nat Biotechnol 19:1134–1140
Sasson I, Taylo H (2008) Stem cells and the pathogenesis of endometriosis. Ann NY Acad Sci 1127:106–115
Schwab KE, Hutchinson P, Gargett CE (2008) Identification of surface markers for prospective isolation of human endometrial stromal colony-forming cells. Hum Reprod 23:934–943
Sher F, Balasubramaniyan V, Boddeke E et al (2008) Oligodendrocyte differentiation and implantation: new insights for remyelinating cell therapy. Curr Opin Neurol 21:607–614
Uccelli A, Mancardi G (2010) Stem cell transplantation in multiple sclerosis. Curr Opin Neurol 23:218–225
Wang Y, Deng Z, Lai X et al (2005) Differentiation of human bone marrow stromal cells into neural-like cells induced by sodium ferulate in vitro. Cell Mol Immunol 2:225–229
Wolff E, Gao X, Yao K et al (2010) Endometrial stem cell transplantation restores dopamine production in a Parkinson disease model. J Cell Mol Med 15:747–755
Zhang HT, Fan J, Cai YQ et al (2010) Human Wharton’s jelly cells can be induced to differentiate into growth factor-secreting oligodendrocyte progenitor-like cells. Differentiation 79:15–20
Zhou Q, Wang S, Anderson DJ (2000) Identification of a novel family of oligodendrocyte lineage-specific basic helix-loop-helix transcription factors. Neuron 25:331–343
Acknowledgments
We thank the research assistant of the Tehran University of Medical Sciences for supporting this work and the Research Center for Science and Technology in Medicine and Iranian Council of Stem Cell Technology.
Conflict of Interest
None.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ebrahimi-Barough, S., Kouchesfahani, H.M., Ai, J. et al. Differentiation of Human Endometrial Stromal Cells into Oligodendrocyte Progenitor Cells (OPCs). J Mol Neurosci 51, 265–273 (2013). https://doi.org/10.1007/s12031-013-9957-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12031-013-9957-z