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Immunohistochemical detection of the Raf kinase inhibitor protein in nonneoplastic gastric tissue and gastric cancer tissue

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Abstract

Expression of the Raf kinase inhibitor protein (RKIP), a metastasis suppressor, is high in normal tissues, low in primary cancers, and lowest or absent in metastatic cancers. Here, we studied the expression of RKIP in nonneoplastic gastric tissue and gastric cancer tissue by immunohistochemistry (IHC) and evaluated its role in the genesis and metastasis of gastric cancer. RKIP immunoreactivity was evaluated in 40 samples of nonneoplastic gastric tissues and 75 samples of gastric cancer tissues. Among the 40 samples of nonneoplastic gastric tissue, 35 (87.5%) were positive for RKIP expression and 5 (12.5%) were negative; in the 75 samples of primary gastric cancer tissue, 39 (52%) were positive for RKIP expression and 36 (48%) were negative. Among 26 samples of metastatic lymph node tissues, 5 (19.2%) were positive for RKIP expression and 21 (80.8%) were negative. RKIP expression level was highest in nonneoplastic gastric tissue, low in primary gastric cancer tissue, and lowest or undetectable in metastatic gastric cancer tissue. Our data suggest that RKIP may play a role in the genesis and metastasis of gastric cancer.

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Acknowledgements

This work was supported by Scientific Research Projects of Sichuan Provincial Health Department (No:080216).

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Authors and Affiliations

  1. Department of Surgery, LuZhou Medical College, LuZhou, Sichuan, People’s Republic of China

    Jin Wang & Dong Wang

  2. Department of Oncology, MianYang Central Hospital, MianYang, Sichuan, People’s Republic of China

    Jin Wang, Bing Yao, Yu Zhang, Li Hui, Tang-Zhi Dai & Xiao-Bo Du

  3. Department of Pathology, MianYang Central Hospital, MianYang, Sichuan, People’s Republic of China

    Yong-Hong Yang, An-Qun Wang, Gang Xie, Gang Feng & Zong-Shu Cheng

  4. Department of Surgery, MianYang Central Hospital, MianYang, Sichuan, People’s Republic of China

    Xiao-Bo Du & Dong Wang

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  1. Jin Wang
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  12. Dong Wang
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Correspondence to Xiao-Bo Du or Dong Wang.

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Wang, J., Yang, YH., Wang, AQ. et al. Immunohistochemical detection of the Raf kinase inhibitor protein in nonneoplastic gastric tissue and gastric cancer tissue. Med Oncol 27, 219–223 (2010). https://doi.org/10.1007/s12032-009-9194-z

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  • DOI: https://doi.org/10.1007/s12032-009-9194-z

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