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Postmortem diagnosis of fatal hypothermia/hyperthermia by spectrochemical analysis of plasma

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Abstract

Postmortem diagnosis of extreme-weather-related deaths is a challenging forensic task. Here, we present a state-of-the-art study that employed attenuated total reflection (ATR) Fourier transform infrared (FTIR) spectroscopy in combination with Chemometrics for postmortem diagnosis of fatal hypothermia/hyperthermia by biochemical investigation of plasma in rats. The results of principal component analysis (PCA) and spectral analysis revealed that plasma samples from the fatal hypothermia, fatal hyperthermia, and control groups, are substantially different from each other based on the spectral variations associated with the lipid, carbohydrate and nucleic acid components. Two partial least squares-discriminant analysis (PLS-DA) classification models (hypothermia-nonhypothermia and hyperthermia-nonhyperthermia binary models) with a 100% accuracy rate were constructed. Subsequently, internal cross-validation was performed to assess the robustness of these two models, which resulted in 98.1 and 100% accuracy. Ultimately, classification predictions of 42 unknown plasma samples were performed by these two models, and both models achieved 100% accuracy. Additionally, our results demonstrated that hemolysis and postmortem hypothermic/hyperthermic effects did not weaken the prediction ability of these two classification models. In summary, this work demonstrates ATR-FTIR spectroscopy’s great potential for postmortem diagnosis of fatal hypothermia/hyperthermia.

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Acknowledgements

This project was supported by grants from the National Key R&D Program of China (2016YFC0800702), the National Natural Science Foundation of China (81730056, 81722027, 81601645, and 81671869), and the Science and Technology Committee of Shanghai Municipality (17DZ2273200 and 19DZ2292700).

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Author notes

  1. Hancheng Lin and Donghua Zou contributed equally to this work.

Authors and Affiliations

  1. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai, 200063, China

    Hancheng Lin, Donghua Zou, Yiwen Luo, Lei Wang, Zhong Zhang, Ji Zhang, Yijiu Chen, Zhenyuan Wang & Ping Huang

  2. Department of Forensic Pathology, Xi’an Jiaotong University, Xi’an, 710061, China

    Hancheng Lin & Zhenyuan Wang

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  1. Hancheng Lin
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Correspondence to Zhenyuan Wang or Ping Huang.

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This study was approved by the Committee on the Ethics of Animal Experiments of Xi’an Jiaotong University and was conducted strictly according to the recommendations in the Guide for the Care and Use of Laboratory Animals of Xi’an Jiaotong University.

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Lin, H., Zou, D., Luo, Y. et al. Postmortem diagnosis of fatal hypothermia/hyperthermia by spectrochemical analysis of plasma. Forensic Sci Med Pathol 15, 332–341 (2019). https://doi.org/10.1007/s12024-019-00111-8

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  • DOI: https://doi.org/10.1007/s12024-019-00111-8

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