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Increasing the reach of forensic genetics with massively parallel sequencing

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Abstract

The field of forensic genetics has made great strides in the analysis of biological evidence related to criminal and civil matters. More so, the discipline has set a standard of performance and quality in the forensic sciences. The advent of massively parallel sequencing will allow the field to expand its capabilities substantially. This review describes the salient features of massively parallel sequencing and how it can impact forensic genetics. The features of this technology offer increased number and types of genetic markers that can be analyzed, higher throughput of samples, and the capability of targeting different organisms, all by one unifying methodology. While there are many applications, three are described where massively parallel sequencing will have immediate impact: molecular autopsy, microbial forensics and differentiation of monozygotic twins. The intent of this review is to expose the forensic science community to the potential enhancements that have or are soon to arrive and demonstrate the continued expansion the field of forensic genetics and its service in the investigation of legal matters.

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Authors and Affiliations

  1. Center for Human Identification, University of North Texas Health Science Center, 3500 Camp Bowie Blvd, CBH-250, Fort Worth, TX, 76107, USA

    Bruce Budowle, Sarah E. Schmedes & Frank R. Wendt

  2. Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah, Saudi Arabia

    Bruce Budowle

Authors
  1. Bruce Budowle
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  2. Sarah E. Schmedes
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  3. Frank R. Wendt
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Correspondence to Bruce Budowle.

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The authors state that they have no conflicts of interest to declare related to this Review. As this is a Review there was no funding required for its production.

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Budowle, B., Schmedes, S.E. & Wendt, F.R. Increasing the reach of forensic genetics with massively parallel sequencing. Forensic Sci Med Pathol 13, 342–349 (2017). https://doi.org/10.1007/s12024-017-9882-5

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