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MiR-5787 Attenuates Macrophages-Mediated Inflammation by Targeting TLR4/NF-κB in Ischemic Cerebral Infarction

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Abstract

Accumulating studies have suggested the important role of microRNA (miRNA) in ischemic cerebral infarction. However, little is known of the modifying effect of miR-5787, a newly found miRNA, in ischemic cerebral infarction. We aim to elucidate the effect and underlying molecular mechanism of miR-5787 in the pathogenesis of ischemic cerebral infarction. MiR-5787 is demonstrated to be downregulated in peripheral blood mononuclear cells (PBMCs) samples of patients compared with controls, which is negatively associated with inflammatory cytokines of IL-6 and TNF-α in ischemic cerebral infarction. Besides, the expression of miR-5787 is also negatively related to TLR4, which is unregulated in PBMCs of ischemic cerebral infarction patients. Moreover, TLR4 is demonstrated to be a target of miR-5787 by bioinformatics’ analysis and the luciferase reporter assay. In addition, miR-5787 can prevent from the proliferation and migration of macrophages, and attenuate LPS/TLR4-mediated inflammatory response via NF-κB in macrophages. MiR-5787 may be a promising biomarker for ischemic cerebral infarction.

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Acknowledgements

This work was supported by the Brain disease innovation team fund of First People's Hospital of Kunshan (KYC004).

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Author notes

  1. Zhicheng Bao and Shuguang Zhang have contributed equally to this work.

Authors and Affiliations

  1. Department of Neurology, Affiliated Kunshan Hospital of Jiangsu University, Suzhou, Jiangsu, 215300, People’s Republic of China

    Zhicheng Bao

  2. Department of Neurosurgery, Affiliated Kunshan Hospital of Jiangsu University, Suzhou, Jiangsu, 215300, People’s Republic of China

    Shuguang Zhang & Xiaoliang Li

  3. 91 Qianjin West Road, Kunshan City, Suzhou, China

    Xiaoliang Li

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  1. Zhicheng Bao
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  2. Shuguang Zhang
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Correspondence to Xiaoliang Li.

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Bao, Z., Zhang, S. & Li, X. MiR-5787 Attenuates Macrophages-Mediated Inflammation by Targeting TLR4/NF-κB in Ischemic Cerebral Infarction. Neuromol Med 23, 363–370 (2021). https://doi.org/10.1007/s12017-020-08628-w

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