Abstract
microRNA, a family of small non-coding RNA, plays significant roles in regulating gene expression, mainly via binding to the 3′-untranslated region of target genes. Although the role of miRNA in regulating neuroinflammation via the innate immune pathway has been studied, its role in the production of inflammatory mediators during microglial activation is poorly understood. In this study, we investigated the effect of miR-27a on lipopolysaccharide (LPS)-induced microglial inflammation. miR-27a expression was found to be rapidly decreased in microglia by real-time polymerase chain reaction (real-time PCR) after LPS stimulation. Over-expression of miR-27a significantly decreased the production of inflammatory cytokines, such as interleukin-6 (IL-6), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and nitric oxide (NO), whereas knockdown of miR-27a increased the expression of these inflammatory factors. We also demonstrated by loss- and gain-of-function studies that miR-27a directly suppressed the expression of toll-like receptor 4 (TLR4) and interleukin-1 receptor-associated kinase 4 (IRAK4)—a pivotal adaptor kinase in the TLR4/MyD88 signaling pathway—by directly binding their 3′-UTRs: knocking down TLR4 or IRAK4 in microglia significantly decreased TLR4 or IRAK4 expression and inhibited the downstream production of inflammatory mediators. Moreover, the inflammatory cytokines IL-6 and IL-1β were regulated by IRAK4, whereas TNF-α and NO were more dependent on TLR4 activation. Thus, miR-27a might regulate the LPS-induced production of inflammatory cytokines in microglia independently of TLR4 and IRAK4. Taken together, our results suggest that miR-27a is associated with microglial activation and the inflammatory response.
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References
Block ML, Zecca L, Hong JS (2007) Microglia-mediated neuro-toxicity: uncovering the molecular mechanisms. Nat Rev Neurosci 8:57–69
Bozic I, Savic D, Laketa D, Bjelobaba I, Milenkovic I, Pekovic S, Nedeljkovic N, Lavrnja I (2015) Benfotiamine attenuates inflammatory response in LPS stimulated BV-2 microglia. PLoS ONE 10:e0118372
Chen XM, Splinter PL, O’Hara SP, LaRusso NF (2007) A cellular micro-RNA, let-7i, regulates Toll-like receptor 4 expression and contributes to cholangiocyte immune responses against Cryptosporidium parvum infection. J Biol Chem 282:28929–28938
Chen WJ, Yin K, Zhao GJ, Fu YC, Tang CK (2012) The magic and mystery of microRNA-27 in atherosclerosis. Atherosclerosis 222:314–323
Chen Q, Xu J, Li L, Li H, Mao S, Zhang F, Zen K, Zhang CY, Zhang Q (2014) MicroRNA-23a/b and microRNA-27a/b suppress Apaf-1 protein and alleviate hypoxia-induced neuronal apoptosis. Cell Death Dis 5:e1132
Cheng Y, Du L, Jiao H, Zhu H, Xu K, Guo S, Shi Q, Zhao T, Pang F, Jia X, Wang F (2015) Mmu-miR-27a-5p-dependent upregulation of MCPIP1 inhibits the inflammatory response in LPS-induced RAW264.7 macrophage cells. Biomed Res Int 2015:607692
Curtale G, Mirolo M, Renzi TA, Rossato M, Bazzoni F, Locati M (2013) Negative regulation of toll-like receptor 4 signaling by IL-10-dependent microRNA-146b. Proc Natl Acad Sci USA 110:11499–11504
Fitzgerald KA, Palsson-McDermott EM, Bowie AG, Jefferies CA, Mansell AS, Brady G, Brint E, Dunne A, Gray P, Harte MT, McMurray D, Smith DE, Sims JE, Bird TA, O’Neill LA (2001) Mal (MyD88-adapter-like) is required for toll-like receptor-4 signal transduction. Nature 413:78–83
Freeman LC, Ting JP (2016) The pathogenic role of the inflammasome in neurodegenerative diseases. J Neurochem 136(Suppl 1):29–38
Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR (1982) Analysis of nitrate, nitrite, and [15 N] nitrate in biological fluids. Anal Biochem 126:131–138
Gupta S, Ellis SE, Ashar FN, Moes A, Bader JS, Zhan J, West AB, Arking DE (2014) Transcriptome analysis reveals dysregulation of innate immune response genes and neuronal activity-dependent genes in autism. Nat Commun 5:5748
Hailer NP (2008) Immunosuppression after traumatic or ischemic CNS damage: it is neuroprotective and illuminates the role of microglial cells. Prog Neurobiol 84:211–233
Hirota T, Date Y, Nishibatake Y, Takane H, Fukuoka Y, Taniguchi Y, Burioka N, Shimizu E, Nakamura H, Otsubo K, Ieiri I (2012) Dihydropyrimidine dehydrogenase (DPD) expression is negatively regulated by certain microRNAs in human lung tissues. Lung Cancer 77:16–23
Jahav SP, Kamath SP, Choolani M, Lu J, Dheen ST (2014) microRNA-200b modulates microglia-mediated neuroinflammation via the cJun/MAPK pathway. J Neurochem 130:388–401
Jennewein C, von Knethen A, Schmid T, Brüne B (2010) MicroRNA-27b contributes to lipopolysaccharide-mediated peroxisome proliferator-activated receptor γ (PPARγ) mRNA destabilization. J Biol Chem 285:11846–11853
Jiang C, Zhu W, Xu J, Wang B, Hou W, Zhang R, Zhong N, Ning Q, Han Y, Yu H, Sun J, Meng L, Lu S (2014) MicroRNA-26a negatively regulates toll-like receptor 3 expression of rat macrophages and ameliorates pristane induced arthritis in rats. Arthritis Res Ther 16:R9
Kang BK, Kim MK, Kim SY, Lee SJ, Choi YW, Choi BT, Shin HK (2015) Anti-neuroinflammatory effects of Uncaria sinensis in LPS-Stimulated BV2 microglia cells and focal cerebral ischemic mice. Am J Chin Med 43:1099–1115
Kong H, Omran A, Ashhab MU, Gan N, Peng J, He F, Wu L, Deng X, Yin F (2014) Changes in microglial inflammation-related and brain-enriched MicroRNAs expressions in response to in vitro oxygen-glucose deprivation. Neurochem Res 39:233–243
Kong H, Yin F, He F, Omran A, Li L, Wu T, Wang Y, Peng J (2015) The effect of miR-132, miR-146a, and miR-155 on MRP8/TLR4-induced astrocyte-related inflammation. J Mol Neurosci 57:28–37
Kumar A, Bhatia HS, de Oliveira AC, Fiebich BL (2015) microRNA-26a modulates inflammatory response induced by toll-like receptor 4 stimulation in microglia. J Neurochem 135:1189–1202
Landais I, Pelton C, Streblow D, DeFilippis V, McWeeney S, Nelson JA (2015) Human cytomegalovirus miR-UL112-3p targets TLR2 and modulates the TLR2/IRAK1/NFκB signaling pathway. PLoS Pathog 11:e1004881
Lehnardt S, Massillon L, Follett P, Jensen FE, Ratan R, Rosenberg PA, Volpe JJ, Vartanian T (2003) Activation of innate immunity in the CNS triggers neurodegeneration through a toll-like receptor 4-dependent pathway. Proc Natl Acad Sci USA 100:8514–8519
Li D, Wang X, Lan X, Li Y, Liu L, Yi J, Li J, Sun Q, Wang Y, Li H, Zhong N, Holmdahl R, Lu S (2015a) miR-144 elicits proinflammatory cytokine production by targeting toll-like receptor 2 in nonalcoholic steatohepatitis of high-fat-diet-induced metabolic syndrome E3 rats. Mol Cell Endocrinol 402:1–12
Li XQ, Lv HW, Wang ZL, Tan WF, Fang B, Ma H (2015b) MiR-27a ameliorates inflammatory damage to the blood-spinal cord barrier after spinal cord ischemia: reperfusion injury in rats by downregulating TICAM-2 of the TLR4 signaling pathway. J Neuroinflamm 12:25
Liu P, Zhao H, Wang R, Wang P, Tao Z, Gao L, Yan F, Liu X, Yu S, Ji X, Luo Y (2015) MicroRNA-424 protects against focal cerebral ischemia and reperfusion injury in mice by suppressing oxidative stress. Stroke 46:513–519
Lv Y, Qian Y, Fu L (2015) Prediction of regulating network of innate immune signaling molecule hsa-miR-181a in stroke development based on bioinformatics analysis. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 31:1042–1047 (in Chinese)
Min S, Li L, Zhang M, Zhang Y, Liang X, Xie Y, He Q, Li Y, Sun J, Liu Q, Jiang X, Zhang Y, Che Y, Yang R (2012) TGF-β-associated miR-27a inhibits dendritic cell-mediated differentiation of Th1 and Th17 cells by TAB 3, p38 MAPK, MAP2K4 and MAP2K7. Genes Immun 13:621–631
Nahid MA, Satoh M, Chan EK (2011) Mechanistic role of microRNA-146a in endotoxin-induced differential cross-regulation of TLR signaling. J Immunol 186:1723–1734
Nakajima K, Kohsaka S (2004) Microglia: neuroprotective and neurotrophic cells in the central nervous system. Curr Drug Targets Cardiovasc Haematol Disord 4:65–84
Ni J, Wang X, Chen S, Liu H, Wang Y, Xu X, Cheng J, Jia J, Zhen X (2015) MicroRNA let-7c-5p protects against cerebral ischemia injury via mechanisms involving the inhibition of microglia activation. Brain Behav Immun 49:75–85
Nie M, Yu S, Peng S, Fang Y, Wang H, Yang X (2015) miR-23a and miR-27a promote human granulosa cell apoptosis by targeting SMAD5. Biol Reprod 93:98
Picard C, Puel A, Bonnet M, Ku CL, Bustamante J, Yang K, Soudais C, Dupuis S, Feinberg J, Fieschi C, Elbim C, Hitchcock R, Lammas D, Davies G, Al-Ghonaium A, Al-Rayes H, Al-Jumaah S, Al-Hajjar S, Al-Mohsen IZ, Frayha HH, Rucker R, Hawn TR, Aderem A, Tufenkeji H, Haraguchi S, Day NK, Good RA, Gougerot-Pocidalo MA, Ozinsky A, Casanova JL (2003) Pyogenic bacterial infections in humans with IRAK-4 deficiency. Science 299:2076–2079
Quinn SR, O’Neill LA (2014) The role of microRNAs in the control and mechanism of action of IL-10. Curr Top Microbiol Immunol 380:145–155
Sharma N, Verma R, Kumawat KL, Basu A, Singh SK (2015) miR-146a suppresses cellular immune response during Japanese encephalitis virus JaOArS982 strain infection in human microglial cells. J Neuroinflamm 18(12):30
Suzuki N, Suzuki S, Duncan GS, Millar DG, Wada T, Mirtsos C, Takada H, Wakeham A, Itie A, Li S, Penninger JM, Wesche H, Ohashi PS, Mak TW, Yeh WC (2002) Severe impairment of interleukin-1 and toll-like receptor signalling in mice lacking IRAK-4. Nature 416:750–756
Teng GG, Wang WH, Dai Y, Wang SJ, Chu YX, Li J (2013) Let-7b is involved in the inflammation and immune responses associated with Helicobacter pylori infection by targeting toll-like receptor 4. PLoS ONE 8:e56709
Wang K, Xie D, Xie J, Wan Y, Ma L, Qi X, Yang S (2015) MiR-27a regulates Wnt/beta-catenin signaling through targeting SFRP1 in glioma. NeuroReport 26:695–702
Zhang L, Dong LY, Li YJ, Hong Z, Wei WS (2012a) The microRNA miR-181c controls microglia-mediated neuronal apoptosis by suppressing tumor necrosis factor. J Neuroinflamm 9:211
Zhang L, Dong LY, Li YJ, Hong Z, Wei WS (2012b) miR-21 represses FasL in microglia and protects against microglia mediated neuronal cell death following hypoxia/ischemia. Glia 60:1888–1895
Zhang L, Li YJ, Wu XY, Hong Z, Wei WS (2015) MicroRNA-181c negatively regulates the inflammatory response in oxygen-glucose-deprived microglia by targeting toll-like receptor 4. J Neurochem 132:713–723
Zhao H, Tao Z, Wang R, Liu P, Yan F, Li J, Zhang C, Ji X, Luo Y (2014) MicroRNA-23a-3p attenuates oxidative stress injury in a mouse model of focal cerebral ischemia-reperfusion. Brain Res 1592:65–72
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Yan-ni Lv, Ai-jun Ou-yang, and Long-sheng Fu have no competing interests to declare.
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Lv, Yn., Ou-yang, Aj. & Fu, Ls. MicroRNA-27a Negatively Modulates the Inflammatory Response in Lipopolysaccharide-Stimulated Microglia by Targeting TLR4 and IRAK4. Cell Mol Neurobiol 37, 195–210 (2017). https://doi.org/10.1007/s10571-016-0361-4
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DOI: https://doi.org/10.1007/s10571-016-0361-4