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Effects and Mechanism Analysis of Vascular Endothelial Growth Factor and Salvianolic Acid B on 125I-Low Density Lipoprotein Permeability of the Rabbit Aortary Endothelial Cells

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Abstract

Atherosclerosis is the common pathological basis of cardiovascular and cerebrovascular disease. This study aimed to investigate the effects of vascular endothelial growth factor (VEGF) and salvianolic acid B (SalB) on the permeability of the rabbit aortary endothelial cells (RAECs) and to figure out the possible underlying molecular mechanisms. The extravasation of 125I-low density lipoprotein (125I-LDL) through the RAECs was significantly increased by VEGF and decreased by SalB. Meanwhile, the tight junction-associated proteins occludin and claudin-5 were found downregulated by VEGF and the caveolae structure proteins caveolin-1 and caveolin-2 upregulated, which were abolished by the infusion of SalB. In addition, a marked increase in levels of cGMP and protein kinase G-1 (PKG-1) as well as activation of nuclear factor-κB (NF-κB) p65 were found after VEGF infusion, which were attenuated by SalB. This study demonstrates that VEGF and SalB can alter the LDL permeability of the RAECs by a paracellular pathway (downregulation of occludin and claudin-5) and a transcellular pathway (upregulation of caveolin-1 and caveolin-2), in which the cGMP/PKG/NF-κB signal pathway is possibly involved. The experimental results provide a new method and basic knowledge of prevention and treatment for cardiovascular and cerebrovascular disease.

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Acknowledgments

Supported by Liaoning Provincial Natural Science Foundation (NO. 201102140) and by Program for Excellent Talents in Liaoning Province, China (NO. LJQ2013088).

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Authors and Affiliations

  1. Emergency Department, Shanghai Tenth People’s Hospital, Shanghai, 200072, China

    Jianming Ba, Hu Peng, Yanqing Chen & Yong Gao

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  1. Jianming Ba
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  2. Hu Peng
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  4. Yong Gao
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Correspondence to Yong Gao.

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Ba, J., Peng, H., Chen, Y. et al. Effects and Mechanism Analysis of Vascular Endothelial Growth Factor and Salvianolic Acid B on 125I-Low Density Lipoprotein Permeability of the Rabbit Aortary Endothelial Cells. Cell Biochem Biophys 70, 1533–1538 (2014). https://doi.org/10.1007/s12013-014-0089-z

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  • DOI: https://doi.org/10.1007/s12013-014-0089-z

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